TTL Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

TTL Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

CloneNo.

2E5F8

Kat-Nr. : 66076-1-Ig

Synonyme

2E5F8, EC:6.3.2.25, Tubulin tyrosine ligase, tubulin tyrosine ligase, Tubulin--tyrosine ligase

---

• 

  WB analysis using 66076-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 66076-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66076-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66076-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66076-1-Ig(TTL antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 66076-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66076-1-Ig(TTL antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, fetales humanes Hirngewebe, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, Hausschwein-Hirngewebe, Kaninchenhirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Produktinformation

66076-1-Ig bindet in WB, IHC, IF, ELISA TTL und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TTL fusion protein Ag4708
• Vollständiger Name: tubulin tyrosine ligase
• Berechnetes Molekulargewicht: 377 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC036819
• Gene symbol: TTL
• Gene ID (NCBI): 150465
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.

Publikationen

Species	Application	Title
human	IF	J Mol Cell Biol; Tubulin detyrosination promotes human trophoblast syncytium formation.; Authors - Rui Wang
human		Carcinogenesis; Generation of focal mutations and large genomic deletions in the pancreas using inducible in vivo genome editing.; Authors - Amrendra Mishra
		Methods Cell Biol; Dissecting the role of the tubulin code in mitosis.; Authors - Luísa T Ferreira
	WB	Cell Rep; Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.; Authors - Ana C Almeida ; KD Validated
mouse	IF	J Cell Sci; Efficient axonal transport of endolysosomes relies on the balanced ratio of microtubule tyrosination and detyrosination; Authors - Anja Konietzny
human	WB	Nat Commun; α-tubulin detyrosination fine-tunes kinetochore-microtubule attachments; Authors - Hugo Girão ; KO Validated