Beta Tubulin Polyklonaler Antikörper

Beta Tubulin Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 10063-2-AP

Synonyme

Tubulin beta-2B chain, TUBB2B

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  WB analysis using 10063-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10063-2-AP (Beta Tubulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 10063-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10063-2-AP (Beta Tubulin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 10063-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10063-2-AP (Beta Tubulin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 10063-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10063-2-AP (Beta Tubulin antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 10063-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10063-2-AP (Beta Tubulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

10063-2-AP bindet in WB, IHC, IP, CoIP, ELISA Beta Tubulin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Beta Tubulin fusion protein Ag0108
• Vollständiger Name: tubulin, beta 2B
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50kda
• GenBank-Zugangsnummer: BC001352
• Gene symbol: Beta Tubulin
• Gene ID (NCBI): 347733
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

There are five tubulins in human cells. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules.

Publikationen

Species	Application	Title
human	CoIP	Biol Psychiatry; Molecular networks of DYX1C1 gene show connection to neuronal migration genes and cytoskeletal proteins.; Authors - Kristiina Tammimies
mouse	WB	J Am Soc Nephrol; Tubular von Hippel-Lindau knockout protects against rhabdomyolysis-induced AKI.; Authors - Michael Fähling
human	WB	Nucleic Acids Res; Hypoxia-induced gene expression results from selective mRNA partitioning to the endoplasmic reticulum.; Authors - Jonas J Staudacher
mouse	WB	Acta Physiol (Oxf); A dual role of miR-22 in rhabdomyolysis-induced acute kidney injury.; Authors - S Mathia
		Ecotoxicol Environ Saf; Cinchophen induces RPA1 related DNA damage and apoptosis to impair ENS development of zebrafish; Authors - Jing Wang
human	WB,IP	Cancer Biol Med; IL13Rα1 prevents a castration resistant phenotype of prostate cancer by targeting hexokinase 2 for ubiquitin-mediated degradation.; Authors - Tingting Feng

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