TUBB3-specific/TUJ1 Monoklonaler Antikörper

TUBB3-specific/TUJ1 Monoklonal Antikörper für WB, IF-P, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(2)

CloneNo.

1F8G10

Rezensionen (1)

Kat-Nr. : CL594-66375

Synonyme

TUBB3, TUBB3-specific, TUJ1, beta tubulin, Tubulin beta 3 chain

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  WB analysis using CL594-66375

  Various lysates were subjected to SDS PAGE followed by western blot with CL594-66375 (TUBB3-specific/TUJ1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse brain using CL594-66375

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-66375 (TUBB3-specific antibody) at dilution of 1:50.

• 

  IF Staining of mouse brain using CL594-66375

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-66375 (TUBB3-specific antibody) at dilution of 1:50.

• 

  IF Staining of mouse brain using CL594-66375

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 TUBB3-specific antibody (CL594-66375, Clone: 1F8G10 ) at dilution of 1:200.

• 

  FC experiment of SH-SY5Y using CL594-66375

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TUBB3-specific (CL594-66375, Clone:1F8G10) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Neuro-2a-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in FC (Intra): SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

CL594-66375 bindet in WB, IHC, IF-P, FC (Intra) TUBB3-specific/TUJ1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tubulin, beta 3
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: NM_001197181
• Gene symbol: TUBB3
• Gene ID (NCBI): 10381
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms. And the antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Publikationen

Species	Application	Title
mouse	IF	Sci Rep; Sexual dimorphic impacts of systemic vincristine on lower urinary tract function.; Authors - Nao Iguchi
mouse	IHC	Exp Cell Res; A parasympathetic neurotransmitter induces myoepithelial cell differentiation during salivary gland development.; Authors - Yuki Shindo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Victoria (Verified Customer) (10-15-2021)

Good IF detection of differentiating neurons in tissue at a concentration of 1:100.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: E3 chick neural tube. FA fixed.