TWEAKR/CD266 Rekombinanter Antikörper

TWEAKR/CD266 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241646F11

Kat-Nr. : 98382-2-RR

Synonyme

TNFRSF12, TWEAK R, CD266, FGF-inducible 14, Fgfrp2

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  FC experiment of Transfected HEK-293T using 98382-2-RR

  1x10^6 HEK-293T cells or TWEAKR/CD266 transfected HEK-293T cells were stained with 0.25 ug Anti-Mouse TWEAK R/TNFRSF12 Rabbit RecAb (98382-2-RR, Clone:241646F11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of Transfected HEK-293T using 98382-2-RR

  1x10^6 HEK-293T cells(left) or TWEAK R/TNFRSF12 transfected HEK-293T cells(right) were stained with 0.25 ug Anti-Mouse TWEAK R/TNFRSF12 Rabbit RecAb (98382-2-RR, Clone:241646F11)(red) , or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98382-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98382-2-RR against Mouse TWEAKR/CD266 were performed. The affinity constant is 0.983 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98382-2-RR bindet in FC TWEAKR/CD266 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TWEAKR/CD266 fusion protein Eg1838
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 12a
• Berechnetes Molekulargewicht: 14kDa
• GenBank-Zugangsnummer: NM_013749.2
• Gene symbol: Tnfrsf12a
• Gene ID (NCBI): 27279
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

TWEAKR (also known as CD266, FN14, TNFRSF12A) is a member of the TNF receptor superfamily which is activated by its ligand, the cytokine TWEAK (TNFSF12). TWEAKR is the smallest member of the TNFR superfamily. TweakR was first described as an FGF-inducible gene that played a role in fibroblast adhesion and migration. Subsequently, the induction of TweakR expression by other growth factors and/or upon tissue injury was observed in multiple cell types, including hepatocytes, endothelial cells, adipocytes, and cardiomyocytes. (PMID: 23073510, PMID: 24409185)