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TWIST1-specific Polyklonaler Antikörper

TWIST1-specific Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF/ICC, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(192)

Kat-Nr. : 25465-1-AP

Synonyme

TWIST1, ACS3, bHLHa38, BPES2, BPES3



Geprüfte Anwendungen

Erfolgreiche Detektion in WBU-251-Zellen, A549-Zellen, MDA-MB-231-Zellen, PC-3-Zellen, Raji-Zellen, SH-SY5Y-Zellen
Erfolgreiche Detektion in IHChumanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Pankreaskarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCL02-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

25465-1-AP bindet in WB, IHC, IF/ICC, CoIP, ChIP, ELISA TWIST1-specific und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus, Ratte, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TWIST1-specific fusion protein Ag21924
Vollständiger Name twist homolog 1 (Drosophila)
Berechnetes Molekulargewicht 202 aa, 21 kDa
Beobachtetes Molekulargewicht25-30 kDa
GenBank-ZugangsnummerBC036704
Gene symbol TWIST1
Gene ID (NCBI) 7291
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Twist-related protein 1 (TWIST1), also named as BHLHA38, is 202 amino acid protein, which contains one bHLH domain. TWIST1 localizes in the nucleus and acts as a transcriptional regulator. TWIST1 inhibits monogenesis by sequestrating E proteins and inhibits tans-activation by MEF2. TWIST1 is expressed in subset of mesodermal cells. The antibody reacts specifically with Twist1 protein. The calculated molecular weight of TWIST1 is 21 kDa, but the observed molecular weight is about 30 kDa due to the post-translational modification(PMID: 30733340).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for TWIST1-specific antibody 25465-1-APProtokoll herunterladen
IHC protocol for TWIST1-specific antibody 25465-1-APProtokoll herunterladenl
IF protocol for TWIST1-specific antibody 25465-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Sci Adv

Phospholipid remodeling is critical for stem cell pluripotency by facilitating mesenchymal-to-epithelial transition.

Authors - Yi Wu
mouseWB,IHC

Theranostics

Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma

Authors - Lu-Ning Qin
humanWB

J Exp Clin Cancer Res

ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK

Authors - Li Cui
mouseWB,IHC

Int J Biol Macromol

Fucoidan/chitosan layered PLGA nanoparticles with melatonin loading for inducing intestinal absorption and addressing triple-negative breast cancer progression

Authors - Yu-Wei Yen
humanWB,IHC

Int J Nanomedicine

Combined miR-181a-5p and Ag Nanoparticles are Effective Against Oral Cancer in a Mouse Model

Authors - Guoqiang Xu
humanIHC

Clin Transl Med

FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression.

Authors - Lei Miao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Angie (Verified Customer) (07-26-2025)

WB showed a band below 37 kDa at dilution of 1:1000 incubated overnight at 4°C. Secondary antibody: Donkey-anti-rabbit (Alexa Fluor 680) at 1:20000 dilution incubated at room temperature for 1 hour.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: A549
TWIST1-specific Antibody Western Blot validation (1:1000 dilution) in A549 (Cat no:25465-1-AP)
FH

Pramod Bahadur (Verified Customer) (02-27-2024)

We are missing the vials so I dont have lot number.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: PC3
TWIST1-specific Antibody Western Blot validation (1:1000 dilution) in PC3 (Cat no:25465-1-AP)