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TWIST1-specific Polyklonaler Antikörper
TWIST1-specific Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus und mehr (2)
Anwendung
WB, IHC, IF/ICC, CoIP, ChIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 25465-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | U-251-Zellen, A549-Zellen, MDA-MB-231-Zellen, PC-3-Zellen, Raji-Zellen, SH-SY5Y-Zellen |
Erfolgreiche Detektion in IHC | humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Pankreaskarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | L02-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:300-1:1200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 8 publications below |
WB | See 175 publications below |
IHC | See 30 publications below |
IF | See 10 publications below |
CoIP | See 2 publications below |
ChIP | See 5 publications below |
Produktinformation
25465-1-AP bindet in WB, IHC, IF/ICC, CoIP, ChIP, ELISA TWIST1-specific und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human, Maus, Ratte, Zebrafisch |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | TWIST1-specific fusion protein Ag21924 |
Vollständiger Name | twist homolog 1 (Drosophila) |
Berechnetes Molekulargewicht | 202 aa, 21 kDa |
Beobachtetes Molekulargewicht | 25-30 kDa |
GenBank-Zugangsnummer | BC036704 |
Gene symbol | TWIST1 |
Gene ID (NCBI) | 7291 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Twist-related protein 1 (TWIST1), also named as BHLHA38, is 202 amino acid protein, which contains one bHLH domain. TWIST1 localizes in the nucleus and acts as a transcriptional regulator. TWIST1 inhibits monogenesis by sequestrating E proteins and inhibits tans-activation by MEF2. TWIST1 is expressed in subset of mesodermal cells. The antibody reacts specifically with Twist1 protein. The calculated molecular weight of TWIST1 is 21 kDa, but the observed molecular weight is about 30 kDa due to the post-translational modification(PMID: 30733340).
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for TWIST1-specific antibody 25465-1-AP | Protokoll herunterladen |
IHC protocol for TWIST1-specific antibody 25465-1-AP | Protokoll herunterladenl |
IF protocol for TWIST1-specific antibody 25465-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Sci Adv Phospholipid remodeling is critical for stem cell pluripotency by facilitating mesenchymal-to-epithelial transition. | ||
Theranostics Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma | ||
J Exp Clin Cancer Res ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK | ||
Int J Biol Macromol Fucoidan/chitosan layered PLGA nanoparticles with melatonin loading for inducing intestinal absorption and addressing triple-negative breast cancer progression | ||
Int J Nanomedicine Combined miR-181a-5p and Ag Nanoparticles are Effective Against Oral Cancer in a Mouse Model | ||
Clin Transl Med FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Angie (Verified Customer) (07-26-2025) | WB showed a band below 37 kDa at dilution of 1:1000 incubated overnight at 4°C. Secondary antibody: Donkey-anti-rabbit (Alexa Fluor 680) at 1:20000 dilution incubated at room temperature for 1 hour.
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FH Pramod Bahadur (Verified Customer) (02-27-2024) | We are missing the vials so I dont have lot number.
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