Thymidylate synthase Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Thymidylate synthase Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

27

Kat-Nr. : 15047-1-AP

Synonyme

HsT422, Thymidylate synthase, thymidylate synthetase, TMS, TS, TSase, TYMS

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Galerie der Validierungsdaten

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  WB analysis using 15047-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15047-1-AP

  WB result of Thymidylate synthase antibody (15047-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Thymidylate synthase transfected HEK-293 cells.

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  WB analysis of NIH/3T3 using 15047-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15047-1-AP (Thymidylate synthase antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 15047-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15047-1-AP

  IP Result of anti-Thymidylate synthase (IP:15047-1-AP, 3ug; Detection:15047-1-AP 1:600) with HeLa cells lysate 3200ug.

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  IHC staining of human endometrial cancer using 15047-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 15047-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15047-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15047-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15047-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 15047-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Thymidylate synthase (15047-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, HEK-293-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Endometriumkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 21 publications below
• IHC: See 5 publications below

Produktinformation

15047-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Thymidylate synthase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Thymidylate synthase fusion protein Ag7027
• Vollständiger Name: thymidylate synthetase
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 32-36 kDa
• GenBank-Zugangsnummer: BC002567
• Gene symbol: TYMS
• Gene ID (NCBI): 7298
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.

Publikationen

Species	Application	Title
human	WB	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui
		Nat Commun; CETSA screening identifies known and novel thymidylate synthase inhibitors and slow intracellular activation of 5-fluorouracil.; Authors - Helena Almqvist
human	WB	Cancer Res; 5-Fluorouracil Enhances the Antitumor Activity of the Glutaminase Inhibitor CB-839 against PIK3CA-Mutant Colorectal Cancers.; Authors - Yiqing Zhao
human	WB	Cell Rep; The mTORC1 Signaling Network Senses Changes in Cellular Purine Nucleotide Levels.; Authors - Gerta Hoxhaj
human	WB	Cell Chem Biol; CETSA interaction proteomics define specific RNA-modification pathways as key components of fluorouracil-based cancer drug cytotoxicity.; Authors - Ying Yu Liang
human	WB	Oxid Med Cell Longev; lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients.; Authors - Guibin Liang ; KD Validated

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