Talin-1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

Talin-1 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240294G5

Kat-Nr. : 82856-18-RR

Synonyme

TLN, talin1, talin 1, KIAA1027, ILWEQ

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  WB analysis of HeLa using 82856-18-RR

  WB result of Talin-1 antibody (82856-18-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Talin-1 transfected HeLa cells.

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  WB analysis using 82856-18-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82856-18-RR (Talin-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82856-18-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82856-18-RR (Talin-1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 82856-18-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82856-18-RR (Talin-1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 82856-18-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-18-RR, Clone: 240294G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of A549 using 82856-18-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-18-RR, Clone: 240294G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A549 using 82856-18-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-18-RR, Clone: 240294G5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), (CL594-Phalloidin, red).

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  FC experiment of HeLa using 82856-18-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Tln1 Recombinant Antibody (82856-18-RR, Clone:240294G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of A549 using 82856-18-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Talin-1 Recombinant Antibody (82856-18-RR, Clone: 240294G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82856-18-RR

  Biolayer interferometry (BLl) kinetic assays of 82856-18-RR against Human TLN1 were performed. The affinity constant is 0.923 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, C6-Zellen, HeLa-Zellen, HepG2-Zellen, HUVEC-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen
• Erfolgreiche Detektion in FC (Intra): A549-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82856-18-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Talin-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Talin-1 fusion protein Ag5367
• Vollständiger Name: talin 1
• Berechnetes Molekulargewicht: 2541 aa, 270 kDa
• Beobachtetes Molekulargewicht: ~270 kDa
• GenBank-Zugangsnummer: BC042923
• Gene symbol: Talin-1
• Gene ID (NCBI): 7094
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).