TOM22 Monoklonaler Antikörper

TOM22 Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

1C2B11

Kat-Nr. : CL555-66562

Synonyme

TOMM22, 1C2B11, 1C9 2, hTom22, MST065

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  IF Staining of HepG2 using CL555-66562

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 Tom22 antibody (CL555-66562, Clone: 1C2B11 ) at dilution of 1:200.

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  FC experiment of HEK-293 using CL555-66562

  1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®555-conjugated Tom22 Monoclonal antibody (CL555-66562, Clone:1C2B11)(red), or 0.4 ug CoraLite® Plus 555 Mouse IgG2a isotype control (11A1B2) (CL555-66360-2, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-66562 bindet in IF/ICC, FC (Intra) TOM22 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TOM22 fusion protein Ag1797
• Vollständiger Name: translocase of outer mitochondrial membrane 22 homolog (yeast)
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC009363
• Gene symbol: TOMM22/Tom22
• Gene ID (NCBI): 56993
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tom22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises  the  22 kDa  protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).