U2AF35 Monoklonaler Antikörper
U2AF35 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Maus / IgG2c
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Konjugation
Unkonjugiert
CloneNo.
2B7F11
Kat-Nr. : 60289-1-Ig
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HeLa-Zellen, COLO 320-Zellen, HEK-293-Zellen, Raji-Zellen, Ramos-Zellen |
Erfolgreiche IP | HeLa-Zellen |
Erfolgreiche Detektion in IHC | humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | HEK-293-Zellen, HepG2-Zellen |
Erfolgreiche Detektion in FC (Intra) | Ramos-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:500-1:2000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:1000-1:4000 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 6 publications below |
Produktinformation
60289-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA U2AF35 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human |
Wirt / Isotyp | Maus / IgG2c |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | U2AF35 fusion protein Ag0399 |
Vollständiger Name | U2 small nuclear RNA auxiliary factor 1 |
Berechnetes Molekulargewicht | 28 kDa |
Beobachtetes Molekulargewicht | 35-40 kDa |
GenBank-Zugangsnummer | BC001923 |
Gene symbol | U2AF1 |
Gene ID (NCBI) | 7307 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
U2 auxiliary factor (U2AF), comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2 (RNU2) small nuclear RNA auxiliary factor 1 (U2AF1,synonyms: RN, FP793, U2AF35, U2AFBP, RNU2AF1)is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. The U2AF1 gene is localized to chromosome 21q22.3, which is the critical region for three diseases, progressive myoclonus epilepsy, autoimmune polyglandular disease type 1, and one form of bipolar affective disorder. The calculated molecular weight of U2AF35 is 28 kDa, but the modified U2AF35 protein is about 35-40 kDa.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for U2AF35 antibody 60289-1-Ig | Protokoll herunterladen |
IHC protocol for U2AF35 antibody 60289-1-Ig | Protokoll herunterladenl |
IF protocol for U2AF35 antibody 60289-1-Ig | Protokoll herunterladen |
IP protocol for U2AF35 antibody 60289-1-Ig | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Nat Commun SPF45/RBM17-dependent, but not U2AF-dependent, splicing in a distinct subset of human short introns. | ||
Mol Neurodegener Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing. | ||
Nucleic Acids Res Stoichiometries of U2AF35, U2AF65 and U2 snRNP reveal new early spliceosome assembly pathways. | ||
Cancer Res Mutant U2AF1-induced mis-splicing of mRNA translation genes confers resistance to chemotherapy in acute myeloid leukemia | ||
Nat Commun Metabolic labeling based methylome profiling enables functional dissection of histidine methylation in C3H1 zinc fingers |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Wael (Verified Customer) (08-28-2025) | The first lane is the ladder and the rest are samples from Hek293 cells (Whole cell lysate). Major band appears between 37 and 25 KD markers (closer to the 37D), as expected.
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