UAP1 Monoklonaler Antikörper

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• KD/KO Validated

UAP1 Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

2C7B2

Kat-Nr. : CL594-67545

Synonyme

AntigenX, Antigen X, AGX1, AGX 2, AgX

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  IF Staining of PC-3 using CL594-67545

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using CoraLite®594 UAP1 antibody (CL594-67545, Clone: 2C7B2 ) at dilution of 1:200.

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  FC experiment of PC-3 using CL594-67545

  1x10^6 PC-3 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated UAP1 Monoclonal antibody (CL594-67545, Clone:2C7B2)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (1F8D3) (CL594-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen
• Erfolgreiche Detektion in FC (Intra): PC-3-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67545 bindet in IF/ICC, FC (Intra) UAP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: UAP1 fusion protein Ag9513
• Vollständiger Name: UDP-N-acteylglucosamine pyrophosphorylase 1
• Berechnetes Molekulargewicht: 505 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57-59 kDa
• GenBank-Zugangsnummer: BC009377
• Gene symbol: UAP1
• Gene ID (NCBI): 6675
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UAP1 can convert UTP and GlcNAc-1-P into UDP-GlcNAc, it can also convert UTP and GalNAc-1-P into UDP-GalNAc. There are three isoforms of UAP1, among which isoform  AGX1  has 2 to 3 times higher activity towards GalNAc-1-P, while isoform  AGX2  has 8 times more activity towards GlcNAc-1-P. It has been reported that UAP1 is specific and highly expressed in prostate cancer. UAP1 can protect cells against inhibitors of N-linked glycosylation. UAP1 may play an important role in maintaining INS responsiveness. 67545-1-Ig detects 57 and 59 kDa isoforms in SDS-PAGE.(PMID:25241896)