UBE2D1/2/3/4 Polyklonaler Antikörper

UBE2D1/2/3/4 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 28328-1-AP

Synonyme

E2(17)KB2, PUBC1, UBC4, UBC4/5, UBC5B, UBCH4, UBCH5B, UBE2D1, UBE2D2, UBE2D3, UBE2D4, Ubiquitin carrier protein D2, Ubiquitin protein ligase D2

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Galerie der Validierungsdaten

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  WB analysis using 28328-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28328-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28328-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28328-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 28328-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 28328-1-AP (UBE2D2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 28328-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 28328-1-AP (UBE2D2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 28328-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28328-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28328-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28328-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Maushodengewebe, Mausnierengewebe, MCF-7-Zellen, NIH/3T3-Zellen, PC-12-Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

28328-1-AP bindet in WB, IHC, ELISA UBE2D1/2/3/4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBE2D1/2/3/4 fusion protein Ag28736
• Vollständiger Name: ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast)
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 14-17 kDa
• GenBank-Zugangsnummer: BC033349
• Gene symbol: UBE2D2
• Gene ID (NCBI): 7322
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I; Authors - Cheng-Jie Zhou