UBE2F Polyklonaler Antikörper

UBE2F Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 17056-1-AP

Synonyme

NCE2, NEDD8 carrier protein UBE2F, NEDD8 conjugating enzyme 2, NEDD8 protein ligase UBE2F, UBE2F

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Galerie der Validierungsdaten

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  WB analysis using 17056-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17056-1-AP (UBE2F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17056-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17056-1-AP (UBE2F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 17056-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 17056-1-AP (UBE2F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 17056-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17056-1-AP (UBE2F antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 17056-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17056-1-AP (UBE2F antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of NIH/3T3 using 17056-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 17056-1-AP (UBE2F antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, Jurkat-Zellen, Maus-Eierstockgewebe, Ratten-Eierstockgewebe
• Erfolgreiche Detektion in IHC: humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

17056-1-AP bindet in WB, IHC, IF, ELISA UBE2F und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBE2F fusion protein Ag10764
• Vollständiger Name: ubiquitin-conjugating enzyme E2F (putative)
• Berechnetes Molekulargewicht: 185 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC010549
• Gene symbol: UBE2F
• Gene ID (NCBI): 140739
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBE2F(Ubiquitin-conjugating enzyme E2 F) is also named as NCE2(NEDD8-conjugating enzyme 2) and belongs to the ubiquitin-conjugating enzyme family.The canonical uBE2M catalyses the modification of most cullins, whereas uBE2F is specifically responsible for cuL5 neddylation. uBE2F is regulated differently than uBE2M, supporting the idea that neddylation is used to fine-tune ubiquitin-chain formation by different cullin-dependent E3s(PMID:19465892).

Publikationen

Species	Application	Title
human	WB	Nat Commun; Neddylation inhibition induces glutamine uptake and metabolism by targeting CRL3SPOP E3 ligase in cancer cells.; Authors - Qiyin Zhou
mouse	WB	Nat Commun; The Ube2m-Rbx1 neddylation-Cullin-RING-Ligase proteins are essential for the maintenance of Regulatory T cell fitness.; Authors - Di Wu
human	WB	Biochem Biophys Res Commun; Disruption of protein neddylation with MLN4924 attenuates paclitaxel-induced apoptosis and microtubule polymerization in ovarian cancer cells.; Authors - Xiaoling Hong
human	WB	Signal Transduct Target Ther; Neddylation is a novel therapeutic target for lupus by regulating double negative T cell homeostasis; Authors - Yun Zhang