UBE2L6 Polyklonaler Antikörper

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• KD/KO Validated

UBE2L6 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 17278-1-AP

Synonyme

RIG B, UBCH8, UBE2L6, Ubiquitin carrier protein L6, Ubiquitin protein ligase L6

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  WB analysis of Jurkat using 17278-1-AP

  WB result of UBE2L6 antibody (17278-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBE2L6 transfected Jurkat cells.

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  WB analysis of Jurkat using 17278-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17278-1-AP (UBE2L6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17278-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17278-1-AP (UBE2L6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 17278-1-AP

  IP result of anti-UBE2L6 (IP:17278-1-AP, 4ug; Detection:17278-1-AP 1:1000) with Jurkat cells lysate 2000ug.

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  IF Staining of HeLa using 17278-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using UBE2L6 antibody (17278-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Produktinformation

17278-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA UBE2L6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UBE2L6 fusion protein Ag11045
• Vollständiger Name: ubiquitin-conjugating enzyme E2L 6
• Berechnetes Molekulargewicht: 153 aa, 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC032491
• Gene symbol: UBE2L6
• Gene ID (NCBI): 9246
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse,human	WB	Cell Commun Signal; Decursin induces FLT3-ITD acute myeloid leukemia cell apoptosis by increasing the expression of the ubiquitin-conjugase UBE2L6; Authors - Tianxin Zhang
human	WB	Genomics Proteomics Bioinformatics; High VHL Expression Reverses Warburg Phenotype and Enhances Immunogenicity in Kidney Tumor Cells.; Authors - Songbiao Zhu
human	WB	Commun Biol; The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells; Authors - Christopher Noyes ; KD Validated
human	IHC	Anticancer Res; Association of UBE2L6 and ABCB6 Expression With Platinum Resistance in Serous Ovarian Carcinoma; Authors - Mami Shibahara
human	WB	Cell Death Dis; Induced TRIM21 ISGylation by IFN-β enhances p62 ubiquitination to prevent its autophagosome targeting.; Authors - Jie Jin
human	WB	Exp Gerontol; p53 accelerates endothelial cell senescence in diabetic retinopathy by enhancing FoxO3a ubiquitylation and degradation via UBE2L6; Authors - Ying Cheng