UBE2S Monoklonaler Antikörper

UBE2S Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

8C9E6

Kat-Nr. : 66897-1-Ig

Synonyme

8C9E6, E2 EPF, E2EPF, E2-EPF, EC:2.3.2.23

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  WB analysis of HEK-293 using 66897-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66897-1-Ig (UBE2S antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66897-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66897-1-Ig (UBE2S antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66897-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66897-1-Ig (UBE2S antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66897-1-Ig bindet in WB, ELISA UBE2S und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: UBE2S fusion protein Ag5378
• Vollständiger Name: ubiquitin-conjugating enzyme E2S
• Berechnetes Molekulargewicht: 222 aa, 24 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC066948
• Gene symbol: UBE2S
• Gene ID (NCBI): 27338
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).