• Featured Product
  • KD/KO Validated

UCH-L1/PGP9.5 Polyklonaler Antikörper

UCH-L1/PGP9.5 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (4)

Anwendung

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(139)

Kat-Nr. : 14730-1-AP

Synonyme

PGP9.5, UCHL1, UCHL1/PGP9.5, EC:3.4.19.12, Neuron cytoplasmic protein 9.5



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, C6-Zellen, Maushirngewebe, NCI-H1299-Zellen, Neuro-2a-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen
Erfolgreiche IPA549-Zellen
Erfolgreiche Detektion in IHCMaushirngewebe, human intrahepatic cholangiocarcinoma tissue
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PMaushirngewebe
Erfolgreiche Detektion in IF-FroMaushirngewebe
Erfolgreiche Detektion in IF/ICCA549-Zellen, Maushirngewebe
Erfolgreiche Detektion in FC (Intra)C6-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
Immunfluoreszenz (IF)-FROIF-FRO : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14730-1-AP bindet in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, ELISA UCH-L1/PGP9.5 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, Hausschwein, Hund, Maus, Ratte, nicht-humane Primaten
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen UCH-L1/PGP9.5 fusion protein Ag6490
Vollständiger Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Berechnetes Molekulargewicht 25 kDa
Beobachtetes Molekulargewicht 27 kDa
GenBank-ZugangsnummerBC000332
Gene symbol UCHL1
Gene ID (NCBI) 7345
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for UCH-L1/PGP9.5 antibody 14730-1-APProtokoll herunterladen
IHC protocol for UCH-L1/PGP9.5 antibody 14730-1-APProtokoll herunterladenl
IF protocol for UCH-L1/PGP9.5 antibody 14730-1-APProtokoll herunterladen
IP protocol for UCH-L1/PGP9.5 antibody 14730-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIHC

Nature

The gut metabolite indole-3 propionate promotes nerve regeneration and repair.

Authors - Elisabeth Serger
mouseIF

Nat Biotechnol

Whole-body cellular mapping in mouse using standard IgG antibodies

Authors - Hongcheng Mai
mouseWB

Nature

A leptin-BDNF pathway regulating sympathetic innervation of adipose tissue.

Authors - Putianqi Wang
mouseIHC

Cell Metab

The circadian clock time tunes axonal regeneration

Authors - Francesco De Virgiliis
mouseIHC

Cell Stem Cell

Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.

Authors - Brian Lin
mouseIF

Cell Metab

Metabolic stress drives sympathetic neuropathy within the liver.

Authors - Kaili Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Xiaolu (Verified Customer) (06-05-2024)

This antibody works very well on WB. I tried dilution and found that 1:25000 is good enough. Do not reduced the dilution, or you will get a very strong signal.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:25000
  • Cell Tissue Type: Rat cell and Human cell
UCH-L1/PGP9.5 Antibody Western Blot validation (1:25000 dilution) in Rat cell and Human cell (Cat no:14730-1-AP)
FH

Dan (Verified Customer) (10-30-2023)

This antibody works really well and gives a positive signal on HCC1806 cells and MDAMB436 cells and shows negative expression in colorectal cancer cell lines.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Breast cancer and colorectal cancer cell lines
UCH-L1/PGP9.5 Antibody Western Blot validation (1:2000 dilution) in Breast cancer and colorectal cancer cell lines (Cat no:14730-1-AP)
FH

Kathryn (Verified Customer) (07-10-2020)

We used this antibody for immunofluorescence on frozen mouse ovary tissues sections and it is very specific with minimal background (stained in red in the image).

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: mouse ovary
UCH-L1/PGP9.5 Antibody Immunofluorescence, validation (1:100 dilution) in mouse ovary (Cat no:14730-1-AP)
FH

Ula (Verified Customer) (11-11-2019)

The antibody produced beautiful staining in mouse skin treated with PLP fixative.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: paw skin
UCH-L1/PGP9.5 Antibody Immunohistochemistry, validation (1:200 dilution) in paw skin (Cat no:14730-1-AP)
FH

Rachel (Verified Customer) (07-03-2019)

This product binds with high frequency and sensitivity. I would recommend it to any researchers.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse Brain Homogenate