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UCH-L1/PGP9.5 Polyklonaler Antikörper
UCH-L1/PGP9.5 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (4)
Anwendung
WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 14730-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | A549-Zellen, C6-Zellen, Maushirngewebe, NCI-H1299-Zellen, Neuro-2a-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen |
Erfolgreiche IP | A549-Zellen |
Erfolgreiche Detektion in IHC | Maushirngewebe, human intrahepatic cholangiocarcinoma tissue Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF-P | Maushirngewebe |
Erfolgreiche Detektion in IF-Fro | Maushirngewebe |
Erfolgreiche Detektion in IF/ICC | A549-Zellen, Maushirngewebe |
Erfolgreiche Detektion in FC (Intra) | C6-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
Immunfluoreszenz (IF)-P | IF-P : 1:50-1:500 |
Immunfluoreszenz (IF)-FRO | IF-FRO : 1:50-1:500 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 7 publications below |
WB | See 27 publications below |
IHC | See 31 publications below |
IF | See 75 publications below |
ELISA | See 2 publications below |
CoIP | See 5 publications below |
Produktinformation
14730-1-AP bindet in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, ELISA UCH-L1/PGP9.5 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Affe, Hausschwein, Hund, Maus, Ratte, nicht-humane Primaten |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | UCH-L1/PGP9.5 fusion protein Ag6490 |
Vollständiger Name | ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) |
Berechnetes Molekulargewicht | 25 kDa |
Beobachtetes Molekulargewicht | 27 kDa |
GenBank-Zugangsnummer | BC000332 |
Gene symbol | UCHL1 |
Gene ID (NCBI) | 7345 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for UCH-L1/PGP9.5 antibody 14730-1-AP | Protokoll herunterladen |
IHC protocol for UCH-L1/PGP9.5 antibody 14730-1-AP | Protokoll herunterladenl |
IF protocol for UCH-L1/PGP9.5 antibody 14730-1-AP | Protokoll herunterladen |
IP protocol for UCH-L1/PGP9.5 antibody 14730-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Cell Stem Cell Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency. | ||
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Xiaolu (Verified Customer) (06-05-2024) | This antibody works very well on WB. I tried dilution and found that 1:25000 is good enough. Do not reduced the dilution, or you will get a very strong signal.
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FH Dan (Verified Customer) (10-30-2023) | This antibody works really well and gives a positive signal on HCC1806 cells and MDAMB436 cells and shows negative expression in colorectal cancer cell lines.
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FH Kathryn (Verified Customer) (07-10-2020) | We used this antibody for immunofluorescence on frozen mouse ovary tissues sections and it is very specific with minimal background (stained in red in the image).
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FH Ula (Verified Customer) (11-11-2019) | The antibody produced beautiful staining in mouse skin treated with PLP fixative.
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FH Rachel (Verified Customer) (07-03-2019) | This product binds with high frequency and sensitivity. I would recommend it to any researchers.
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