UQCRC1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

UQCRC1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(44)

Rezensionen (2)

Kat-Nr. : 21705-1-AP

Synonyme

Ubiquinol-cytochrome-c reductase complex core protein 1, QCR1, D3S3191, Cytochrome b-c1 complex subunit 1, mitochondrial, Core protein I

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  WB analysis of HeLa using 21705-1-AP

  WB result of UQCRC1 antibody (21705-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UQCRC1 transfected HeLa cells.

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  WB analysis using 21705-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21705-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours.

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  WB analysis of mouse skeletal muscle using 21705-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21705-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 21705-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 21705-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse large intestine using 21705-1-AP

  mouse large intestine tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 21705-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 21705-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21705-1-AP (UQCRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 21705-1-AP

  IP result of anti-UQCRC1 (IP:21705-1-AP, 4ug; Detection:21705-1-AP 1:500) with HeLa cells lysate 1600ug.

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  IHC staining of human colon using 21705-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21705-1-AP (UQCRC1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 21705-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21705-1-AP (UQCRC1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 21705-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UQCRC1 antibody (21705-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe, Jurkat-Zellen, L02-Zellen, Mausnierengewebe, Maus-Dickdarmgewebe, Mauslebergewebe, Maus-Skelettmuskelgewebe, Mausmilzgewebe, Rattenlebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: human normal colon, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 39 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

21705-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA UQCRC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UQCRC1 fusion protein Ag16322
• Vollständiger Name: ubiquinol-cytochrome c reductase core protein I
• Berechnetes Molekulargewicht: 480 aa, 53 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC009586
• Gene symbol: UQCRC1
• Gene ID (NCBI): 7384
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UQCRC1(Cytochrome b-c1 complex subunit 1, mitochondrial), also named as QCR1 and UQCR1, is a ubunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain, may mediate the formation of the complex between cytochromes c and c1. The gene encodes a 53 kDa protein with a 34 amino acids transit peptide. It is associated with apoptosis or inhibition of cancer cell growth.

Publikationen

Species	Application	Title
human	WB	Sci Bull (Beijing); Targeted clearance of mitochondria by an autophagy-tethering compound (ATTEC) and its potential therapeutic effects; Authors - Shuixia Tan
mouse	WB	Biomaterials; Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation.; Authors - Xiao-Tao He
human	IHC	Theranostics; Mitochondrial Protein UQCRC1 is Oncogenic and a Potential Therapeutic Target for Pancreatic Cancer.; Authors - Qing Wang
		Acta Pharmacol Sin; MicroRNA-214 contributes to Ang II-induced cardiac hypertrophy by targeting SIRT3 to provoke mitochondrial malfunction.; Authors - Yan-Qing Ding
human	WB	Int J Nanomedicine; Activation of Dynamin-Related Protein 1 and Induction of Mitochondrial Apoptosis by Exosome-Rifampicin Nanoparticles Exerts Anti-Osteosarcoma Effect; Authors - Wenkai Chen
human	WB	Aging (Albany NY); Celecoxib reverses the glioblastoma chemo-resistance to temozolomide through mitochondrial metabolism.; Authors - Delong Yin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xingyuan (Verified Customer) (06-30-2023)

Quality was alright, had non-specific band under it.

• Applications: Western Blot
• Cell Tissue Type: 293T

UQCRC1 Antibody Western Blot validation ( dilution) in 293T (Cat no:21705-1-AP)

FH

Xingyuan (Verified Customer) (06-30-2023)

Quality was alright, had non-specific band under it.

• Applications: Western Blot
• Cell Tissue Type: 293T