URG4 Polyklonaler Antikörper

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• KD/KO Validated

URG4 Polyklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-11998

Synonyme

URGCP, Up-regulator of cell proliferation, up regulated gene 4, KIAA1507, HBxAg up-regulated gene 4 protein

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  IF Staining of HeLa using CL488-11998

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 URG4 antibody (CL488-11998) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of HEK-293 using CL488-11998

  1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Urg4 Polyclonal Antibody (CL488-11998)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-11998 bindet in IF/ICC, FC (Intra) URG4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: URG4 fusion protein Ag2630
• Vollständiger Name: up-regulated gene 4
• Berechnetes Molekulargewicht: 922 aa, 104 kDa
• Beobachtetes Molekulargewicht: 104 kDa
• GenBank-Zugangsnummer: BC018426
• Gene symbol: URG4
• Gene ID (NCBI): 55665
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

URG4 (upregulated gene 4), also known as upregulator of cell proliferation (URGCP), is an oncogene which is involved in the development and progression of various tumors. URG4 is upregulated in hepatocellular carcinoma (HCC), ovarian cancer, and other types of cancer. Overexpression of URG4 not only results in tumour progression but also in metastasis and recurrence.