• Featured Product
  • KD/KO Validated

USP21 Polyklonaler Antikörper

USP21 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 17856-1-AP

Synonyme

EC:3.4.19.12, PP1490, Ubiquitin carboxyl-terminal hydrolase 21, Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, HeLa-Zellen, HepG2-Zellen, HuH-7-Zellen, RAW 264.7-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, humanes Pankreasgewebe, Mausherzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCRAW 264.7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

17856-1-AP bindet in WB, IHC, IF/ICC, CoIP, RIP, ELISA USP21 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen USP21 fusion protein Ag12316
Vollständiger Name ubiquitin specific peptidase 21
Berechnetes Molekulargewicht 565 aa, 63 kDa
Beobachtetes Molekulargewicht 63 kDa
GenBank-ZugangsnummerBC090946
Gene symbol USP21
Gene ID (NCBI) 27005
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP21(Ubiquitin carboxyl-terminal hydrolase 21) is also named as USP23.The USPs belong to a subclass of the protein-deubiquitinating enzyme (DUB) superfamily that are categorized into five subclasses based on their ubiquitin-protease domains in the human genome and have been shown to be involved in a broad range of biological activities(PMID:19910467). USP21 is expressed at different levels in the tissues examined. The highest level of USP21 expression is found in the heart, pancreas, and skeletal muscle. USP21 messages could also be detected in the brain, placenta, liver, and kidney but are very low in the Lung through the northern blot(PMID:10799498).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for USP21 antibody 17856-1-APProtokoll herunterladen
IHC protocol for USP21 antibody 17856-1-APProtokoll herunterladenl
IF protocol for USP21 antibody 17856-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Sci Transl Med

Guanosine diphosphate-mannose suppresses homologous recombination repair and potentiates antitumor immunity in triple-negative breast cancer

Authors - Jia-Han Ding
mouseWB

EMBO J

Oligomerization-driven MLKL ubiquitylation antagonizes necroptosis.

Authors - Zikou Liu
humanIHC

Int J Mol Sci

PR-619, a General Inhibitor of Deubiquitylating Enzymes, Diminishes Cisplatin Resistance in Urothelial Carcinoma Cells through the Suppression of c-Myc: An In Vitro and In Vivo Study.

Authors - Fu-Shun Hsu
humanWB

Cell Death Dis

The deubiquitinase USP21 stabilizes MEK2 to promote tumor growth.

Authors - Wenjuan Li
  • KD Validated
humanWB,IF

iScience

TET2 stabilized by deubiquitinase USP21 ameliorates cigarette smoke-induced apoptosis in airway epithelial cells

Authors - Lijuan Luo
humanWB

J Virol

Deubiquitinating Enzyme USP21 Inhibits HIV-1 Replication by Downregulating Tat Expression.

Authors - Wenying Gao
  • KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Damien (Verified Customer) (07-25-2025)

Detection of a protein at the expected size in the cell extracts in WB. Unfortunately, the signal is not loss when USP21 had been knocked down with 2 different pairs of siRNA raised against this protein. This antibody then likely recognizes a non specific band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HEK293T