VAMP3/Cellubrevin Polyklonaler Antikörper

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• KD/KO Validated

VAMP3/Cellubrevin Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA, Blocking

Konjugation

Unkonjugiert

Publikationen(20)

Kat-Nr. : 10702-1-AP

Synonyme

VAMP3, CEB, Cellubrevin, SYB3, Synaptobrevin-3

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  WB analysis of HEK-293 using 10702-1-AP

  WB result of VAMP3/Cellubrevin antibody (10702-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VAMP3/Cellubrevin transfected HEK-293 cells.

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  WB analysis of HEK-293 using 10702-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10702-1-AP (VAMP3/Cellubrevin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10702-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10702-1-AP (VAMP3/Cellubrevin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10702-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10702-1-AP (VAMP3/Cellubrevin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10702-1-AP

  IP result of anti-VAMP3/Cellubrevin (IP:10702-1-AP, 4ug; Detection:10702-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

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  IHC staining of mouse testis using 10702-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10702-1-AP (VAMP3/Cellubrevin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 10702-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10702-1-AP (VAMP3/Cellubrevin antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10702-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10702-1-AP (VAMP3/Cellubrevin antibody at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, MCF-7-Zellen, Maushirngewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 16 publications below
• IHC: See 2 publications below
• IF: See 8 publications below

Produktinformation

10702-1-AP bindet in WB, IHC, IF, IP, ELISA, Blocking VAMP3/Cellubrevin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VAMP3/Cellubrevin fusion protein Ag1156
• Vollständiger Name: vesicle-associated membrane protein 3 (cellubrevin)
• Berechnetes Molekulargewicht: 11 kDa
• Beobachtetes Molekulargewicht: 11-17 kDa
• GenBank-Zugangsnummer: BC005941
• Gene symbol: VAMP3
• Gene ID (NCBI): 9341
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VAMP3, also named as cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration.

Publikationen

Species	Application	Title
mouse	Blocking	Adv Sci (Weinh); Engineered Cell-Derived Microparticles Bi2Se3/DOX@MPs for Imaging Guided Synergistic Photothermal/Low-Dose Chemotherapy of Cancer.; Authors - Dongdong Wang
human	WB,IF	Adv Sci (Weinh); Early Endosomes Act as Local Exocytosis Hubs to Repair Endothelial Membrane Damage; Authors - Nikita Raj
human	WB,IF	Nat Commun; KRAS regulation by small non-coding RNAs and SNARE proteins.; Authors - Yonglu Che
rat	WB,IF	Aging Cell; Knockdown of astrocytic Grin2a aggravates β-amyloid-induced memory and cognitive deficits through regulating nerve growth factor.; Authors - Zunshu Du
human	WB,IF	Oncogene; Syntaxin-6 mediated autophagy confers lenvatinib resistance in hepatocellular carcinoma; Authors - Guo-Pei Zhang
human	IHC	Oncotarget; A novel liver metastasis-correlated protein of pancreatic neuroendocrine neoplasm (PanNEN) discovered by proteomic analysis.; Authors - Mitsuhiro Shimura