VAMP8 Polyklonaler Antikörper

VAMP8 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 15546-1-AP

Synonyme

EDB, Endobrevin, VAMP 8, VAMP8

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Galerie der Validierungsdaten

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  WB analysis using 15546-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15546-1-AP (VAMP8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15546-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15546-1-AP (VAMP8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 15546-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 15546-1-AP (VAMP8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 15546-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15546-1-AP (VAMP8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 15546-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15546-1-AP (VAMP8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse placenta using 15546-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 15546-1-AP (VAMP8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse placenta using 15546-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 15546-1-AP (VAMP8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, PC-12-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, Maus-Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

15546-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA VAMP8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VAMP8 fusion protein Ag7903
• Vollständiger Name: vesicle-associated membrane protein 8 (endobrevin)
• Berechnetes Molekulargewicht: 11 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC001634
• Gene symbol: VAMP8
• Gene ID (NCBI): 8673
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VAMP8, also named as endobrevin, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE (soluble NSF-attachment protein receptor) superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP8 is involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. It is required for dense-granule secretion in platelets and plays a role in regulated enzyme secretion in pancreatic acinar cells. VAMP8 is also involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells.

Publikationen

Species	Application	Title
human	IF	J Extracell Vesicles; Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion; Authors - Chuqi Liu
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
rat	WB	Arch Toxicol; Mystery of methamphetamine-induced autophagosome accumulation in hippocampal neurons: loss of syntaxin 17 in defects of dynein-dynactin driving and autophagosome-late endosome/lysosome fusion.; Authors - Huaisha Xu
human	WB	Ecotoxicol Environ Saf; Weakened interaction of ATG14 and the SNARE complex blocks autophagosome-lysosome fusion contributes to fluoride-induced developmental neurotoxicity.; Authors - Yuanli Zhang
rat	WB	Stem Cell Res Ther; Microtubule destabilization caused by silicate via HDAC6 activation contributes to autophagic dysfunction in bone mesenchymal stem cells.; Authors - Zheng Li
human	WB	Nutrients; Improving Lipophagy by Restoring Rab7 Cycle: Protective Effects of Quercetin on Ethanol-Induced Liver Steatosis.; Authors - Hongkun Lin