VCP Polyklonaler Antikörper

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• KD/KO Validated

VCP Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(53)

Kat-Nr. : 10736-1-AP

Synonyme

15S Mg(2+) ATPase p97 subunit, IBMPFD, p97, TER ATPase, TERA, valosin containing protein, VCP

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  WB analysis of U2OS using 10736-1-AP

  U2OS (WT and VCP KD) lysates prepared with RIPA buffer, 10μg protein loaded. 10736-1-AP incubated at 1:15000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis using 10736-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10736-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 10736-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10736-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lung using 10736-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10736-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10736-1-AP

  IP result of anti-VCP (IP:10736-1-AP, 4ug; Detection:10736-1-AP 1:1000) with HeLa cells lysate 1505ug.

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  IP experiment of mouse brain using 10736-1-AP

  IP result of anti-VCP (IP:10736-1-AP, 4ug; Detection:10736-1-AP 1:300) with mouse brain tissue lysate 2400 ug.

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  IHC staining of human gliomas using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10736-1-AP (VCP Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human gliomas using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10736-1-AP (VCP Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human gliomas using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, humanes Hirngewebe, Maushirngewebe, Mauslungengewebe, Maus-Thymusgewebe, NIH/3T3-Zellen, Rattenhirngewebe, Rattenlungengewebe, SH-SY5Y-Zellen, U2OS-Zellen
• Erfolgreiche IP: HeLa-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 49 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below

Produktinformation

10736-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA VCP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VCP fusion protein Ag1002
• Vollständiger Name: valosin-containing protein
• Berechnetes Molekulargewicht: 89 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC007562
• Gene symbol: VCP
• Gene ID (NCBI): 7415
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).

Publikationen

Species	Application	Title
human	WB	Cell; TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival; Authors - Pauline Lascaux
mouse	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
human	WB	J Clin Invest; Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders; Authors - Huilun Helen Wang
human	WB	Sci Adv; Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.; Authors - Yuan Wang
human	WB	Nat Commun; Analysis of Zika virus capsid-Aedes aegypti mosquito interactome reveals pro-viral host factors critical for establishing infection.; Authors - Rommel J Gestuveo ; KD Validated
human	WB	Cell Rep; hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility; Authors - Xiaoli Wang