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VDAC1/Porin Polyklonaler Antikörper

VDAC1/Porin Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (7)

Anwendung

WB, IHC, IF-P, IF-Fro, FC (Intra), CoIP, ELISA, PLA

Konjugation

Unkonjugiert

Publikationen(203)

Kat-Nr. : 55259-1-AP

Synonyme

Porin, VDAC1, hVDAC1, Plasmalemmal porin, PORIN 31 HL


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  • Western Blot (WB) analysis of HeLa cells using VDAC1/Porin Polyclonal antibody (55259-1-AP)

    WB analysis of HeLa cells using 55259-1-AP

    WB result of VDAC1 antibody (55259-1-AP, 1:2000) with si-Control and si-VDAC1 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using VDAC1/Porin Polyclonal antibody (55259-1-AP)

    WB analysis using 55259-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using VDAC1/Porin Polyclonal antibody (55259-1-AP)

    WB analysis of HepG2 using 55259-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using VDAC1/Porin Polyclonal antibody (55259-1-AP)

    WB analysis of human skeletal muscle using 55259-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using VDAC1/Porin Polyclonal antibody (55259-1-AP)

    WB analysis using 55259-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using VDAC1/Porin Polyclonal antibody (55259-1-AP)

    WB analysis using 55259-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using VDAC1/Porin Polyclonal antibody (55259-1-AP)

    WB analysis of A431 using 55259-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using VDAC1/Porin Polyclonal antibody (55259-1-AP)

    WB analysis of mouse heart using 55259-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using VDAC1/Porin Polyclonal antibody (55259-1-AP)

    WB analysis using 55259-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using VDAC1/Porin Polyclonal antibody (55259-1-AP)

    WB analysis of rat liver using 55259-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using VDAC1/Porin Polyclonal antibody (55259-1-AP)

    WB analysis of mouse liver using 55259-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using VDAC1/Porin Polyclonal antibody (55259-1-AP)

    IHC staining of human heart using 55259-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using VDAC1/Porin Polyclonal antibody (55259-1-AP)

    IHC staining of human heart using 55259-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using VDAC1/Porin Polyclonal antibody (55259-1-AP)

    IHC staining of human liver using 55259-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using VDAC1/Porin Polyclonal antibody (55259-1-AP)

    IHC staining of human liver using 55259-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using VDAC1/Porin Polyclonal antibody (55259-1-AP)

    IF Staining of human liver cancer using 55259-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using VDAC1/Porin antibody (55259-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using VDAC1/Porin Polyclonal antibody (55259-1-AP)

    IF Staining of mouse liver using 55259-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse liver tissue using VDAC1/Porin antibody (55259-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using VDAC1/Porin Polyclonal antibody (55259-1-AP)

    FC experiment of HepG2 using 55259-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human VDAC1/Porin (55259-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

"VDAC1/Porin Antibodies" Comparison

View side-by-side comparison of VDAC1/Porin antibodies from other vendors to find the one that best suits your research needs.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBRAW 264.7-Zellen, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Skelettmuskelgewebe, Maushirngewebe, Mausherzgewebe, Mauslebergewebe, NIH/3T3-Zellen, Rattenhirngewebe, Rattenherzgewebe, Rattenlebergewebe, ROS1728-Zellen
Erfolgreiche Detektion in IHChumanes Herzgewebe, humanes Lebergewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-Phumanes Leberkarzinomgewebe
Erfolgreiche Detektion in IF-FroMauslebergewebe
Erfolgreiche Detektion in FC (Intra)HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:6000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
Immunfluoreszenz (IF)-FROIF-FRO : 1:50-1:500
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

55259-1-AP bindet in WB, IHC, IF-P, IF-Fro, FC (Intra), CoIP, ELISA, PLA VDAC1/Porin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, Ente, hamster, Hausschwein, Huhn, Hund, Maus, Ratte, Rind
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name voltage-dependent anion channel 1
Berechnetes Molekulargewicht 31 kDa
Beobachtetes Molekulargewicht 31 kDa
GenBank-ZugangsnummerNM_003374
Gene symbol Porin
Gene ID (NCBI) 7416
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This antibody is specific to VDAC1.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for VDAC1/Porin antibody 55259-1-APProtokoll herunterladen
IHC protocol for VDAC1/Porin antibody 55259-1-APProtokoll herunterladenl
IF protocol for VDAC1/Porin antibody 55259-1-APProtokoll herunterladen
FC protocol for VDAC1/Porin antibody 55259-1-APDownload protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell

IRGQ-mediated autophagy in MHC class I quality control promotes tumor immune evasion

Authors - Lina Herhaus
humanWB

Nat Commun

MYG1 drives glycolysis and colorectal cancer development through nuclear-mitochondrial collaboration

Authors - Jianxiong Chen
humanWB

Mol Cell

Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology

Authors - Gayathri Muthukumar

Mol Cell

Hexokinase 1 cellular localization regulates the metabolic fate of glucose.

Authors - Adam De Jesus
monkeyWB

Nat Commun

An ESCRT-dependent step in fatty acid transfer from lipid droplets to mitochondria through VPS13D-TSG101 interactions.

Authors - Jingru Wang
humanWB

Nucleic Acids Res

The human tRNA taurine modification enzyme GTPBP3 is an active GTPase linked to mitochondrial diseases.

Authors - Gui-Xin Peng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Ying (Verified Customer) (04-22-2021)

good antibody. works very well with mouse cell lines.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
VDAC1/Porin Antibody Western Blot validation (1:2000 dilution) in (Cat no:55259-1-AP)