VEGFR1/Flt-1 Rekombinanter Antikörper

VEGFR1/Flt-1 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

242311D5

Kat-Nr. : 98418-2-RR

Synonyme

Flt1, FLT-1, VEGFR-1, VGFR1

---

• 

  FC experiment of Transfected HEK-293T using 98418-2-RR

  1x10^6 HEK-293T cells or VEGFR1/Flt-1 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat VEGFR1/Flt-1 Rabbit RecAb (98418-2-RR, Clone:242311D5) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293T using 98418-2-RR

  1x10^6 HEK-293T cells or VEGFR1/Flt-1 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat VEGFR1/Flt-1 Rabbit RecAb (98418-2-RR, Clone: 242311D5) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98418-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98418-2-RR against Rat VEGFR1/Flt-1 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98418-2-RR bindet in FC (Intra) VEGFR1/Flt-1 und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: VEGFR1/Flt-1 fusion protein Eg1167
• Vollständiger Name: fms-related tyrosine kinase 1
• Gene symbol: Flt1
• Gene ID (NCBI): 54251
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Vascular endothelial growth factor receptor-1 (VEGFR-1, FLT-1) is a receptor tyrosine kinase belonging to the VEGFR family. VEGF is a key regulator of physiological angiogenesis and has also been implicated in pathological angiogenesis associated with tumors, intraocular neovascular disorders, and other conditions. The biological effects of VEGF are mediated by VEGFR-1 and VEGFR-2. Both the two receptors have seven immunoglobulin-like repeats in the extracellular domain, a single transmembrane region and a tyrosine kinase domain. VEGFR-1 binds VEGFA, PIGF, and VEGFB, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion.