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Vimentin Polyklonaler Antikörper
Vimentin Polyklonal Antikörper für FC, IF, IHC, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (3)
Anwendung
WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA
Konjugation
Unkonjugiert
1390
Kat-Nr. : 10366-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HeLa-Zellen, Jurkat-Zellen, Mausherzgewebe, NIH/3T3-Zellen |
Erfolgreiche Detektion in IHC | humanes Leberkarzinomgewebe, humanes Lebergewebe, humanes Leberzirrhosegewebe, humanes Mammakarzinomgewebe, humanes Tonsillitisgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF | HepG2-Zellen, humanes Mammakarzinomgewebe, humanes Nierengewebe |
Erfolgreiche Detektion in FC | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunhistochemie (IHC) | IHC : 1:2500-1:10000 |
Immunfluoreszenz (IF) | IF : 1:50-1:500 |
Durchflusszytometrie (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
Produktinformation
10366-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA Vimentin und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, hamster, Hausschwein, Maus, Ratte, Rind |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Vimentin fusion protein Ag0489 |
Vollständiger Name | vimentin |
Berechnetes Molekulargewicht | 466 aa, 54 kDa |
Beobachtetes Molekulargewicht | 54 kDa |
GenBank-Zugangsnummer | BC000163 |
Gene symbol | VIM |
Gene ID (NCBI) | 7431 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).
Protokolle
Produktspezifische Protokolle | |
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WB protocol for Vimentin antibody 10366-1-AP | Protokoll herunterladen |
IHC protocol for Vimentin antibody 10366-1-AP | Protokoll herunterladen |
IF protocol for Vimentin antibody 10366-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Cell Res The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8. | ||
Cell Host Microbe Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation | ||
Circ Res Glutamyl-Prolyl-tRNA Synthetase Regulates Proline-Rich Pro-Fibrotic Protein Synthesis During Cardiac Fibrosis. | ||
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Vernon (Verified Customer) (01-29-2024) | Western blot signal is great with 30 sec exposure secondary antibody 1:5000 1hr. at room temp
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FH Tobias (Verified Customer) (06-16-2023) | Works well in IF
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FH Emma (Verified Customer) (02-22-2022) | Works well by IF on FFPE prostate tissue. (Tris-EDTA antigen retrieval).
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FH Yuki (Verified Customer) (01-07-2022) | Consistently works antibody
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FH Hala (Verified Customer) (07-08-2021) | Works veryyyy gooddd
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FH Hala (Verified Customer) (04-11-2021) | very good antibody, works very well
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FH wei (Verified Customer) (04-27-2020) | I tried in C2C12 cells
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FH Patricia (Verified Customer) (02-25-2020) | Works well.
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FH Kishor (Verified Customer) (01-30-2019) | Good results in western blotting but I could not see consistent results in IF
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FH Jaime (Verified Customer) (01-24-2018) |
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