VPS37A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

VPS37A Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 11870-1-AP

Synonyme

ESCRT I complex subunit VPS37A, HCRP1, hVps37A, PQBP2, VPS37A

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Galerie der Validierungsdaten

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  WB analysis using 11870-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11870-1-AP

  WB result of VPS37A antibody (11870-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS37A transfected MCF-7 cells.

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  WB analysis of human liver using 11870-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11870-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11870-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11870-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11870-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11870-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 11870-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11870-1-AP (VPS37A antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 11870-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11870-1-AP (VPS37A antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of human liver using 11870-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using VPS37A antibody (11870-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Lebergewebe, Mauslebergewebe, MCF-7-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Lebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

11870-1-AP bindet in WB, IHC, IF, ELISA VPS37A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS37A fusion protein Ag2453
• Vollständiger Name: vacuolar protein sorting 37 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC022363
• Gene symbol: VPS37A
• Gene ID (NCBI): 137492
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VPS37A, also named as HCRP1, belongs to the VPS37 family. It is a regulator of vesicular trafficking process. VPS37A is a member of the endosomal sorting complex required for transport (ESCRT) system, in upper motor neuron disease. ESCRT has been shown to play a central role in intracellular trafficking, in the maturation of multivesicular bodies and the sorting of ubiquitinated membrane proteins into internal luminal vesicles.

Publikationen

Species	Application	Title
human	WB,IF	Autophagy; Removal of hypersignaling endosomes by simaphagy; Authors - Simona M Migliano ; KO Validated
human	WB	Curr Biol; UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.; Authors - Stefani Flavia F
human	WB	Structure; The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain.; Authors - Monica Agromayor
human	WB,IHC	Cell Death Dis; HCRP-1 regulates EGFR-AKT-BIM-mediated anoikis resistance and serves as a prognostic marker in human colon cancer.; Authors - Feifei Chen ; KD Validated
human	WB	J Cell Sci; The molecular basis for selective assembly of the UBAP1-containing endosome-specific ESCRT-I complex.; Authors - Lydia Wunderley
human	WB	J Med Genet; A founder mutation in Vps37A causes autosomal recessive complex hereditary spastic paraparesis.; Authors - Zivony-Elboum Yifat Y