VPS4A/B Polyklonaler Antikörper

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• KD/KO Validated

VPS4A/B Polyklonal Antikörper für WB, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 17673-1-AP

Synonyme

VPS4B, Cell migration-inducing gene 1 protein, EC:3.6.4.6, MIG1, Protein SKD1

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  WB analysis using 17673-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4A/B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 17673-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 17673-1-AP

  IP result of anti-VPS4A/B (IP:17673-1-AP, 4ug; Detection:17673-1-AP 1:3000) with HeLa cells lysate 1360 ug.

• 

  FC experiment of A431 using 17673-1-AP

  1x10^6 A431 cells were intracellularly stained with 0.25 ug VPS4A/B Polyclonal antibody (17673-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HeLa-Zellen, Maushautgewebe, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

17673-1-AP bindet in WB, IF, FC (Intra), IP, ELISA VPS4A/B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS4A/B fusion protein Ag11610
• Vollständiger Name: vacuolar protein sorting 4 homolog B (S. cerevisiae)
• Berechnetes Molekulargewicht: 444 aa, 49 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC039574
• Gene symbol: VPS4B
• Gene ID (NCBI): 9525
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. Human VPS4 has two orthologous proteins named VPS4A and VPS4B. This antibody can recognize both VPS4A and VPS4B due to their high homology.

Publikationen

Species	Application	Title
human	WB	Cell Res; RAB31 marks and controls an ESCRT-independent exosome pathway.; Authors - Denghui Wei
human	IF	Nat Cell Biol; Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.; Authors - Vincent Mercier
rat	WB,IF	EMBO J; TDP-43 loss of function inhibits endosomal trafficking and alters trophic signaling in neurons.; Authors - Benjamin M Schwenk
		J Cell Biol; ALIX- and ESCRT-III-dependent sorting of tetraspanins to exosomes.; Authors - Jorge Larios
human	WB	J Cell Sci; His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation; Authors - Gabrielle Parkinson
human	WB	Viruses; Characterization of Bovine Foamy Virus Gag Late Assembly Domain Motifs and Their Role in Recruiting ESCRT for Budding.; Authors - Zhaohuan Wang