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Vimentin Monoklonaler Antikörper
Vimentin Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human, Maus, Ratte und mehr (3)
Anwendung
WB, IHC, IF/ICC, IP, CoIP, ELISA
Konjugation
Unkonjugiert
CloneNo.
3H9D1
Kat-Nr. : 60330-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HeLa-Zellen, A549-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, ROS1728-Zellen, U-251-Zellen, U2OS-Zellen |
| Erfolgreiche Detektion in IHC | humanes Appendizitis-Gewebe, humanes Mammakarzinomgewebe, humanes Kolongewebe, humanes Endometriumkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Ovarialkarzinomgewebe, humanes Pankreasgewebe, humanes Nierenzellkarzinomgewebe, humanes Tonsillitisgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | HUVEC-Zellen, HepG2-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunhistochemie (IHC) | IHC : 1:4000-1:16000 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 234 publications below |
| IHC | See 36 publications below |
| IF | See 118 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Produktinformation
60330-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA Vimentin und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Hausschwein, Kaninchen, Maus, Ratte, Zebrafisch |
| Wirt / Isotyp | Maus / IgG1 |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | Vimentin fusion protein Ag0489 |
| Vollständiger Name | vimentin |
| Berechnetes Molekulargewicht | 466 aa, 54 kDa |
| Beobachtetes Molekulargewicht | 55-60 kDa |
| GenBank-Zugangsnummer | BC000163 |
| Gene symbol | VIM |
| Gene ID (NCBI) | 7431 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-G-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for Vimentin antibody 60330-1-Ig | Protokoll herunterladen |
| IHC protocol for Vimentin antibody 60330-1-Ig | Protokoll herunterladenl |
| IF protocol for Vimentin antibody 60330-1-Ig | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Adv Mater Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine | ||
Mol Cancer CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer. | ||
Adv Sci (Weinh) Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis | ||
Acta Pharm Sin B Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1. | ||
J Pineal Res Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics | ||
Theranostics Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Alessandro (Verified Customer) (12-09-2023) | good IF antibody. no unspecific staining
|
FH Aarushi (Verified Customer) (04-15-2023) | Primary antibody worked well after methanol treatment in a dilution of 1:200.
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FH Saba (Verified Customer) (03-03-2022) | The antibody worked perfectly!! The band was sharp and no unspecefic bands were seen.
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