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Vimentin Monoklonaler Antikörper

Vimentin Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(328)

CloneNo.

3H9D1

Kat-Nr. : 60330-1-Ig

Synonyme

VIM, 3H9D1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, A549-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, ROS1728-Zellen, U-251-Zellen, U2OS-Zellen
Erfolgreiche Detektion in IHChumanes Appendizitis-Gewebe, humanes Mammakarzinomgewebe, humanes Kolongewebe, humanes Endometriumkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Ovarialkarzinomgewebe, humanes Pankreasgewebe, humanes Nierenzellkarzinomgewebe, humanes Tonsillitisgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHUVEC-Zellen, HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:20000-1:100000
Immunhistochemie (IHC)IHC : 1:4000-1:16000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

60330-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA Vimentin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Kaninchen, Maus, Ratte, Zebrafisch
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Vimentin fusion protein Ag0489
Vollständiger Name vimentin
Berechnetes Molekulargewicht 466 aa, 54 kDa
Beobachtetes Molekulargewicht 55-60 kDa
GenBank-ZugangsnummerBC000163
Gene symbol VIM
Gene ID (NCBI) 7431
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Vimentin antibody 60330-1-IgProtokoll herunterladen
IHC protocol for Vimentin antibody 60330-1-IgProtokoll herunterladenl
IF protocol for Vimentin antibody 60330-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
ratIF

Adv Mater

Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine

Authors - Peng Lou
humanWB,IP,IF

Mol Cancer

CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer.

Authors - Ya Fan
mouseIF

Adv Sci (Weinh)

Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis

Authors - Bin Liu
mouseWB

Acta Pharm Sin B

Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1.

Authors - Piao Luo
humanWB

J Pineal Res

Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics

Authors - Ye Young Shin
mouseWB

Theranostics

Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma

Authors - Lu-Ning Qin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Alessandro (Verified Customer) (12-09-2023)

good IF antibody. no unspecific staining

  • Applications: Immunofluorescence
  • Cell Tissue Type: Retinal organoids
FH

Aarushi (Verified Customer) (04-15-2023)

Primary antibody worked well after methanol treatment in a dilution of 1:200.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Tanycyte monolayer cell cultures (mouse)
Vimentin Antibody Immunofluorescence validation (1:200 dilution) in Tanycyte monolayer cell cultures (mouse) (Cat no:60330-1-Ig)
FH

Saba (Verified Customer) (03-03-2022)

The antibody worked perfectly!! The band was sharp and no unspecefic bands were seen.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MCF10A cells