• Featured Product
  • KD/KO Validated

Vimentin Monoklonaler Antikörper

Vimentin Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(2)

CloneNo.

3H9D1

Kat-Nr. : CL594-60330

Synonyme

Cell and organelle markers, Cytoskeleton Marker, FLJ36605, VIM, vimentin



Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IFSee 2 publications below

Produktinformation

CL594-60330 bindet in IF/ICC Vimentin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Vimentin fusion protein Ag0489
Vollständiger Name vimentin
Berechnetes Molekulargewicht 466 aa, 54 kDa
GenBank-ZugangsnummerBC000163
Gene symbol VIM
Gene ID (NCBI) 7431
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL594 Vimentin antibody CL594-60330Protokoll herunterladen
FC protocol for CL594 Vimentin antibody CL594-60330Download protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
ratIF

Mater Today Bio

Engineered muscle from micro-channeled PEG scaffold with magnetic Fe3O4 fixation towards accelerating esophageal muscle repair

Authors - Yang Luo
humanIF

Front Cell Dev Biol

Ectopic Endometrial Cell-Derived Exosomal Moesin Induces Eutopic Endometrial Cell Migration, Enhances Angiogenesis and Cytosolic Inflammation in Lesions Contributes to Endometriosis Progression.

Authors - Maidinaimu Abudula