Vinculin Polyklonaler Antikörper

Vinculin Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(134)

Rezensionen (8)

Kat-Nr. : 26520-1-AP

Synonyme

CMD1W, Metavinculin, MV, MVCL, VCL

---

• 

  WB analysis using 26520-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26520-1-AP (Vinculin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26520-1-AP (Vinculin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26520-1-AP (Vinculin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 26520-1-AP (Vinculin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 26520-1-AP (Vinculin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human prostate cancer using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate cancer using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A549 using 26520-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Vinculin antibody (26520-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 26520-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Vinculin antibody (26520-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 26520-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Vinculin (26520-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, MDCK-Zellen, Mausherzgewebe, NIH/3T3-Zellen, PC-12-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe, humanes Mammakarzinomgewebe, humanes Prostatakarzinomgewebe, humanes Skelettmuskelgewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, A549-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:60000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 104 publications below
• IF: See 38 publications below
• IP: See 1 publications below

Produktinformation

26520-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA Vinculin und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Vinculin fusion protein Ag24942
• Vollständiger Name: vinculin
• Berechnetes Molekulargewicht: 1133 aa, 124 kDa
• Beobachtetes Molekulargewicht: 124 kDa
• GenBank-Zugangsnummer: BC039174
• Gene symbol: Vinculin
• Gene ID (NCBI): 7414
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Publikationen

Species	Application	Title
human	WB,IF	Cell Metab; Electron transport chain inhibition increases cellular dependence on purine transport and salvage; Authors - Zheng Wu
human	WB	Blood; Epigenetic Regulation of Non-canonical Menin Targets Modulates Menin Inhibitor Response in Acute Myeloid Leukemia; Authors - Xinyue Zhou
human	WB,IF	ACS Nano; Polyethylenimine-Modified Mesoporous Silica Nanoparticles Induce a Survival Mechanism in Vascular Endothelial Cells via Microvesicle-Mediated Autophagosome Release; Authors - Lu Zhang
human	WB	Mol Cell; Type III CRISPR-mediated flexible RNA excision with engineered guide RNAs; Authors - Yuanfan Sun
mouse	IF	Adv Sci (Weinh); Highly Effective and Noninvasive Near-Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min.; Authors - Mu Li
rabbit	WB,IF	Adv Sci (Weinh); Biomimetic Convex Implant for Corneal Regeneration Through 3D Printing; Authors - Yingni Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Tsimafei (Verified Customer) (09-22-2025)

Excellent and specific antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: U2OS

Vinculin Antibody Western Blot validation (1:10000 dilution) in U2OS (Cat no:26520-1-AP)

FH

Elham (Verified Customer) (06-18-2025)

Human airway smooth muscle cells fixed after 1 h of plating. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X 100. Blocked with 0.1% BSA and incubated with primary antibody overnight at 4C at 1:100 dilution. Following day cells were washed and incubated for 1 h with Alexa 594 at 1:400 dilution.

• Applications: Immunofluorescence, Cell culture
• Primary Antibody Dilution: 1:100

Vinculin Antibody Immunofluorescence,Cell culture validation (1:100 dilution) in (Cat no:26520-1-AP)

FH

Siavash (Verified Customer) (01-17-2025)

The antibody was used for immunofluorescence staining of 2D cultured cells fixed with 1%PFA and permeabilized with Triton X100. Cells were incubated overnight at +4 degrees celcius. The staining was visible in both epifluorescence microscope and confocal fluorescence microscope. Also, used for immunostaining of formalin-fixed paraffin-embedded (FFPE) skin of mouse. The antigen retrieval was citrate buffer. Primary antibody was incubated overnight at +4 degrees celcius.

• Applications: Immunofluorescence
• Primary Antibody Dilution: Cell: 1:300, Tissue: 1:100
• Cell Tissue Type: Human Primary Epidermal Keratinocytes, Mouse Skin

Vinculin Antibody Immunofluorescence validation (Cell: 1:300, Tissue: 1:100 dilution) in Human Primary Epidermal Keratinocytes, Mouse Skin (Cat no:26520-1-AP)

FH

Sammy (Verified Customer) (12-04-2023)

Very good antibody for detection of vinculin by WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: HEKs / HUVEC

Vinculin Antibody Western Blot validation (1 in 1000 dilution) in HEKs / HUVEC (Cat no:26520-1-AP)

FH

Verdiana (Verified Customer) (11-03-2022)

Band at about 140kDa. Great antibody! Bands were visible just after 0.5sec of exposure! Clear bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 in TBST 5% Milk
• Cell Tissue Type: U2OS

Vinculin Antibody Western Blot validation (1:2000 in TBST 5% Milk dilution) in U2OS (Cat no:26520-1-AP)

FH

Bettina (Verified Customer) (08-03-2022)

Antibody worked well!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse pancreatic cancer cell line

Vinculin Antibody Western Blot validation (1:1000 dilution) in Mouse pancreatic cancer cell line (Cat no:26520-1-AP)

FH

Silvia (Verified Customer) (02-14-2022)

Good sharp clear band in western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000

FH

Bhuvanasundar (Verified Customer) (10-12-2021)

Immunofluorescence staining with this antibody worked very well.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Epithelial cell