Vinculin Polyklonaler Antikörper

Vinculin Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

59

Rezensionen (5)

Kat-Nr. : 26520-1-AP

Synonyme

CMD1W, Metavinculin, MVCL, VCL, Vinculin

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Galerie der Validierungsdaten

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  WB analysis using 26520-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26520-1-AP (Vinculin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 26520-1-AP (Vinculin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 26520-1-AP (Vinculin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human prostate cancer using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate cancer using 26520-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26520-1-AP (Vinculin Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 26520-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26520-1-AP (Vinculin antibody) at dilution of 1:600 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL555-phalloidine stains F-actin (red).

• 

  IF Staining of HepG2 using 26520-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 26520-1-AP (Vinculin antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 26520-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Vinculin (26520-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Mausherzgewebe, MDCK-Zellen, NIH/3T3-Zellen, PC-12-Zellen, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe, humanes Mammakarzinomgewebe, humanes Prostatakarzinomgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:300-1:1200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 42 publications below
• IF: See 24 publications below

Produktinformation

26520-1-AP bindet in WB, IHC, IF, FC, ELISA Vinculin und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Vinculin fusion protein Ag24942
• Vollständiger Name: vinculin
• Berechnetes Molekulargewicht: 1133 aa, 124 kDa
• Beobachtetes Molekulargewicht: 117 kDa
• GenBank-Zugangsnummer: BC039174
• Gene symbol: VCL
• Gene ID (NCBI): 7414
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Publikationen

Species	Application	Title
human	WB,IF	ACS Nano; Polyethylenimine-Modified Mesoporous Silica Nanoparticles Induce a Survival Mechanism in Vascular Endothelial Cells via Microvesicle-Mediated Autophagosome Release; Authors - Lu Zhang
mouse	IF	Adv Sci (Weinh); Highly Effective and Noninvasive Near-Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min.; Authors - Mu Li
rabbit	WB,IF	Adv Sci (Weinh); Biomimetic Convex Implant for Corneal Regeneration Through 3D Printing; Authors - Yingni Xu
human	WB	Nat Commun; Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer.; Authors - Kai Yuan
human	IF	Small; Versatile Protein Coronation Approach with Multiple Depleted Serum for Creating Biocompatible, Precision Nanomedicine.; Authors - Chan Wang
human	WB	J Exp Clin Cancer Res; RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization; Authors - Shuang Tao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sammy (Verified Customer) (12-04-2023)

Very good antibody for detection of vinculin by WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: HEKs / HUVEC

Vinculin Antibody Western Blot validation (1 in 1000 dilution) in HEKs / HUVEC (Cat no:26520-1-AP)

FH

Verdiana (Verified Customer) (11-03-2022)

Band at about 140kDa. Great antibody! Bands were visible just after 0.5sec of exposure! Clear bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 in TBST 5% Milk
• Cell Tissue Type: U2OS

Vinculin Antibody Western Blot validation (1:2000 in TBST 5% Milk dilution) in U2OS (Cat no:26520-1-AP)

FH

Bettina (Verified Customer) (08-03-2022)

Antibody worked well!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse pancreatic cancer cell line

Vinculin Antibody Western Blot validation (1:1000 dilution) in Mouse pancreatic cancer cell line (Cat no:26520-1-AP)

FH

Silvia (Verified Customer) (02-14-2022)

Good sharp clear band in western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000

FH

Bhuvanasundar (Verified Customer) (10-12-2021)

Immunofluorescence staining with this antibody worked very well.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Epithelial cell

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C

Vinculin Monoclonal antibody

66305-1-Ig

FAK Monoclonal antibody

66258-1-Ig

FAK Polyclonal antibody

12636-1-AP

Paxillin Polyclonal antibody

10029-1-Ig

Beta Actin Monoclonal antibody

66009-1-Ig

Beta Actin Monoclonal antibody

60008-1-Ig

E-cadherin Monoclonal antibody

60335-1-Ig

E-cadherin Polyclonal antibody

20874-1-AP