WBP2 Polyklonaler Antikörper

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• KD/KO Validated

WBP2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 12030-1-AP

Synonyme

WW domain-binding protein 2, WW domain binding protein 2, WBP-2, WBP 2

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  WB analysis using 12030-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12030-1-AP (WBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12030-1-AP

  WB result of WBP2 antibody (12030-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-WBP2 transfected HEK-293 cells.

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  IP experiment of A375 using 12030-1-AP

  IP result of anti-WBP2 (IP:12030-1-AP, 4ug; Detection:12030-1-AP 1:300) with A375 cells lysate 3600 ug.

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  IHC staining of human breast cancer using 12030-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12030-1-AP (WBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 12030-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12030-1-AP (WBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12030-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12030-1-AP (WBP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 12030-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12030-1-AP (WBP2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A375 using 12030-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using WBP2 antibody (12030-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HEK-293-Zellen, MCF-7-Zellen, MDA-MB-453s-Zellen
• Erfolgreiche IP: A375-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Mammakarzinomgewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

12030-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA WBP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WBP2 fusion protein Ag2661
• Vollständiger Name: WW domain binding protein 2
• Berechnetes Molekulargewicht: 261 aa, 28 kDa
• Beobachtetes Molekulargewicht: 30-40 kDa
• GenBank-Zugangsnummer: BC010616
• Gene symbol: WBP2
• Gene ID (NCBI): 23558
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Redox Biol; WBP2 restrains the lysosomal degradation of GPX4 to inhibit ferroptosis in cisplatin-induced acute kidney injury; Authors - Zebin Deng
mouse	WB	Eur J Immunol; Itch attenuates CD4 T cell proliferation in mice by limiting WBP2 protein stability.; Authors - Natania S Field
mouse	WB,IF	Biol Reprod; WBP2 shares a common location in mouse spermatozoa with WBP2NL/PAWP and like its descendent is a candidate mouse oocyte activating factor.; Authors - Lauren E Hamilton
rat	IF	Biol Reprod; The perforatorium and postacrosomal sheath of rat spermatozoa share common developmental origins and protein constituents†.; Authors - Nicole Protopapas
human	WB, IF	Cell Death Dis; WW domain-binding protein 2 overexpression prevents diet-induced liver steatosis and insulin resistance through AMPKβ1.; Authors - Zhe Zheng
human,mouse	WB,IHC	Cell Death Discov; EIF4A3-mediated circPRKCI expression promotes triple-negative breast cancer progression by regulating WBP2 and PI3K/AKT signaling pathway.; Authors - Xuehui Wang