WDFY3/ALFY Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

WDFY3/ALFY Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 55009-1-AP

Synonyme

WDFY3, WDFY3 / ALFY, Alfy, Autophagy linked FYVE protein, Autophagy-linked FYVE protein

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  IHC staining of rat brain using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 55009-1-AP (WDFY3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55009-1-AP (WDFY3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55009-1-AP (WDFY3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55009-1-AP (WDFY3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55009-1-AP (WDFY3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55009-1-AP (WDFY3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55009-1-AP (WDFY3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55009-1-AP (WDFY3 Antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55009-1-AP (WDFY3 Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Rattenhirngewebe, humanes Mammakarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

55009-1-AP bindet in WB, IHC, IF, ELISA WDFY3/ALFY und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: WD repeat and FYVE domain containing 3
• Berechnetes Molekulargewicht: 395 kDa
• GenBank-Zugangsnummer: NM_014991
• Gene symbol: WDFY3
• Gene ID (NCBI): 23001
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WDFY3, also named as KIAA0993 and Alfy, is a protein which contains WD repeats and an FYVE domain. Multiple alternatively spliced transcript variants have been found for WDFY3, but the full-length nature of some variants has not been defined. The antibody is specific to WDFY3.The band 50kd in WB is a splicing form of WDFY3.

Publikationen

Species	Application	Title
mouse	WB,IHC,IF	Cardiovasc Res; WD40 repeat and FYVE domain containing 3 (Wdfy3) is essential for cardiac development.; Authors - Shasha Zhang ; KO Validated
mouse	IHC,IF	In Vitro Cell Dev Biol Anim; Autophagy-linked FYVE protein (Alfy) promotes autophagic removal of misfolded proteins involved in amyotrophic lateral sclerosis (ALS).; Authors - Huihui Han