WDR1 Polyklonaler Antikörper

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• KD/KO Validated

WDR1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 13676-1-AP

Synonyme

Actin interacting protein 1, AIP1, NORI 1, WD repeat containing protein 1, WD repeat domain 1, WDR1

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Galerie der Validierungsdaten

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  WB analysis using 13676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13676-1-AP (WDR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13676-1-AP

  WB result of WDR1 antibody (13676-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-WDR1 transfected HEK-293 cells.

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  WB analysis of rat skeletal muscle using 13676-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13676-1-AP (WDR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 13676-1-AP

  IP Result of anti-WDR1 (IP:13676-1-AP, 3ug; Detection:13676-1-AP 1:500) with mouse skeletal muscle tissue lysate 3600ug.

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  IHC staining of human testis using 13676-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13676-1-AP (WDR1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 13676-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13676-1-AP (WDR1 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Jurkat-Zellen, Ratten-Skelettmuskelgewebe, U-87-Zellen
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 9 publications below

Produktinformation

13676-1-AP bindet in WB, IP, IHC, ELISA WDR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WDR1 fusion protein Ag4670
• Vollständiger Name: WD repeat domain 1
• Berechnetes Molekulargewicht: 606 aa, 66 kDa
• Beobachtetes Molekulargewicht: 66-70 kDa
• GenBank-Zugangsnummer: BC030541
• Gene symbol: WDR1
• Gene ID (NCBI): 9948
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WDR1, which belongs to the WD repeat AIP1 family, also known as AIP1, is a 606 amino acid protein that localizes to the cytoskeleton. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, mostly including a trp-asp at the C-terminal end. WDR1 induces the disassembly of actin filaments in conjunction with ADF/cofilin family proteins (PMID:15629458, 27557945, 29751004). WDR1 mutations may impair the ability of WDR1 to regulate cofilin-mediated actin depolymerization, and are associated with periodic fever, immunodeficiency, and thrombocytopenia syndrome (PMID: 27557945,27994071,29751004).

Publikationen

Species	Application	Title
mouse	WB	Cell Death Dis; AIP1-mediated actin disassembly is required for postnatal germ cell migration and spermatogonial stem cell niche establishment.; Authors - J Xu
human	WB	J Biol Chem; Trp-Asp (WD) repeat domain 1 is Essential for Mouse Peri-implantation Development and Regulates Cofilin Phosphorylation.; Authors - Yi Xiao
mouse	WB	Am J Pathol; A Cardiomyocyte-Specific Wdr1 Knockout Demonstrates Essential Functional Roles for Actin Disassembly during Myocardial Growth and Maintenance In Mice.; Authors - Baiyin Yuan ; KO Validated
human,mouse	WB	Mol Cells; WD Repeat Domain 1 Deficiency Inhibits Neointima Formation in Mice Carotid Artery by Modulation of Smooth Muscle Cell Migration and Proliferation.; Authors - JiSheng Hu ; KO Validated
human	WB	Int J Biol Sci; WDR1 Promotes Cell Growth and Migration and Contributes to Malignant Phenotypes of Non-small Cell Lung Cancer through ADF/cofilin-mediated Actin Dynamics.; Authors - Baiyin Yuan ; KD Validated
mouse	WB	Dev Biol; WDR1-regulated actin dynamics is required for outflow tract and right ventricle development.; Authors - Jisheng Hu ; KO Validated