WEE1 Polyklonaler Antikörper

WEE1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 29474-1-AP

Synonyme

DKFZp686I18166, FLJ16446, WEE1, WEE1 homolog (S. pombe), Wee1 like protein kinase, WEE1A, Wee1A kinase, WEE1hu

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Galerie der Validierungsdaten

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  WB analysis using 29474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29474-1-AP (WEE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

29474-1-AP bindet in WB, IF, ELISA WEE1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WEE1 fusion protein Ag30194
• Vollständiger Name: WEE1 homolog (S. pombe)
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 72 kDa,110 kDa
• GenBank-Zugangsnummer: BC051831
• Gene symbol: WEE1
• Gene ID (NCBI): 7465
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WEE1 kinase is a serine-threonine kinase that regulates G2/M checkpoint transition. WEE1 triggers G2/M arrest through inhibitory phosphorylation on Tyr15 of CDK1 (Cdc2) and preventing entry into mitosis to allow DNA repair during DNA damage (PMID: 27427153).The predicted native molecular weight for Wee1 based on the intact human Wee1 cDNA sequence is 72 kDa, whereas the 95-110 kDa protein is a highly modified product (PMID: 7568188). Others have shown that Wee1 is 70-72 kDa and 49-55 kDa (PMID: 12214061).

Publikationen

Species	Application	Title
mouse	IF	Nucleic Acids Res; DIS3L2 ribonuclease degrades terminal-uridylated RNA to ensure oocyte maturation and female fertility; Authors - Di Wu
human	WB	J Cell Mol Med; Venetoclax enhances DNA damage induced by XPO1 inhibitors: A novel mechanism underlying the synergistic antileukaemic effect in acute myeloid leukaemia.; Authors - Hanxi Yu
mouse	WB	Reprod Toxicol; High-dose zearalenone exposure disturbs G2/M transition during mouse oocyte maturation.; Authors - Yi-Ming Ji
human	WB	Anal Cell Pathol (Amst); miR-138-5p Inhibits the Growth and Invasion of Glioma Cells by Regulating WEE1.; Authors - Jianwu Gong