WNT10B Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

WNT10B Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

CloneNo.

1G11E4

Kat-Nr. : 67210-1-Ig

Synonyme

1G11E4, Protein Wnt 10b, Protein Wnt 12, Protein Wnt-10b, Protein Wnt-12

---

• 

  WB analysis using 67210-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67210-1-Ig (WNT10B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 67210-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 67210-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 67210-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 67210-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 67210-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 67210-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 67210-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 67210-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67210-1-Ig (WNT10B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67210-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67210-1-Ig (WNT10B antibody), at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NCCIT-Zellen, humanes Herzgewebe, MCF-7-Zellen, Mausherzgewebe, NCI-H1299-Zellen, Hausschwein-Herzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe, humanes Herzgewebe, Mausherzgewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

67210-1-Ig bindet in WB, IHC, IF/ICC, ELISA WNT10B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: WNT10B fusion protein Ag17114
• Vollständiger Name: wingless-type MMTV integration site family, member 10B
• Berechnetes Molekulargewicht: 389 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC096353
• Gene symbol: WNT10B
• Gene ID (NCBI): 7480
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Wnt10b is a member of the Wnt ligand gene family that encodes for secreted proteins, which activate the ancient and highly conserved Wnt signalling cascade. This antibody can be detected a band of 43 kDa.

Publikationen

Species	Application	Title
human	WB,IF	Pharmacol Res; Sinomenine hydrochloride suppresses the stemness of breast cancer stem cells by inhibiting Wnt signaling pathway through down-regulation of WNT10B.; Authors - Xiaomei Li ; KD Validated
human	WB	J Cancer; MPP7 mediates EMT via Wnt/β-catenin pathway to promote polarity changes in epithelial ovarian cancer cells; Authors - Chunlin Tao
mouse	WB	Nat Commun; FoxO3 controls cardiomyocyte proliferation and heart regeneration by regulating Sfrp2 expression in postnatal mice; Authors - Jing-Bo Xia
	WB	Int J Biol Macromol; Xylooligosaccharides alleviate the carbohydrate-enriched diet-induced intestinal barrier dysfunction in carp Megalobrama amblycephala by promoting intestinal development, immunity and gut microbiota; Authors - Wei-Liang Chen
human	WB	Heliyon; MAP7 drives EMT and cisplatin resistance in ovarian cancer via wnt/β-catenin signaling; Authors - Qingqing Chen