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WT1 Polyklonaler Antikörper

WT1 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23235-1-AP

Synonyme

GUD, WAGR, Wilms tumor 1, Wilms tumor protein, WIT 2, WT1, WT33

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of mouse kidney tissue using WT1 Polyclonal antibody (23235-1-AP)

    WB analysis of mouse kidney using 23235-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23235-1-AP (WT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMausnierengewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

23235-1-AP bindet in WB,ELISA WT1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen WT1 fusion protein Ag19541
Vollständiger Name Wilms tumor 1
Berechnetes Molekulargewicht 449 aa, 49 kDa, 57 kDa
GenBank-ZugangsnummerBC032861
Gene symbol WT1
Gene ID (NCBI) 7490
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. Function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.