WWP2 Polyklonaler Antikörper

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• KD/KO Validated

WWP2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 12197-1-AP

Synonyme

AIP2, WW domain containing protein 2, WWP2, WWp2 like

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Galerie der Validierungsdaten

• 

  WB analysis of mouse liver using 12197-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12197-1-AP (WWP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12197-1-AP

  WB result of WWP2 antibody (12197-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WWP2 transfected HepG2 cells.

• 

  WB analysis of A549 using 12197-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12197-1-AP (WWP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 12197-1-AP

  IP result of anti-WWP2 (IP:12197-1-AP, 4ug; Detection:12197-1-AP 1:1000) with A549 cells lysate 800 ug.

• 

  IHC staining of human pancreas cancer using 12197-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12197-1-AP (WWP2 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, A549-Zellen, HepG2-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

12197-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA WWP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WWP2 fusion protein Ag2835
• Vollständiger Name: WW domain containing E3 ubiquitin protein ligase 2
• Berechnetes Molekulargewicht: 870 aa, 99 kDa
• Beobachtetes Molekulargewicht: 110-115 kDa
• GenBank-Zugangsnummer: BC013645
• Gene symbol: WWP2
• Gene ID (NCBI): 11060
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WWP2(WW domain-containing protein 2) is also named as AIP2 and belongs to the NEDD4-like protein family. It functions as an E3 ubiquitin ligase for PTEN that plays a vital role in tumour-cell survival and is also involved in regulating transcription, embryonic stem-cell fate, cellular transport and T-cell activation processes(PMID:21532586).WWP2, which specifically interacted with ES cell-specific transcription factor Oct-4 and promoted its ubiquitination both in vitro and in vivo, is a ubiquitously expressed protein and exists in both cytoplasmic and nuclear compartments in ES cells(PMID:15047715).

Publikationen

Species	Application	Title
	WB,IF	Cardiovasc Diabetol; Down-regulation of WWP2 aggravates Type 2 diabetes mellitus-induced vascular endothelial injury through modulating ubiquitination and degradation of DDX3X; Authors - Shilong You ; KD Validated
human	WB	Cell Death Differ; Selective targeting of ubiquitination and degradation of PARP1 by E3 ubiquitin ligase WWP2 regulates isoproterenol-induced cardiac remodeling.; Authors - Naijin Zhang ; KD Validated
mouse	WB	Redox Biol; Role of WW domain E3 ubiquitin protein ligase 2 in modulating ubiquitination and Degradation of Septin4 in oxidative stress endothelial injury.; Authors - Naijin Zhang
human	WB	Br J Pharmacol; Costunolide represses hepatic fibrosis through WWP2-mediated Notch3 degradation.; Authors - Mao-Xu Ge
mouse	WB,IHC,IF	J Dent Res; WWP2 Promotes Odontoblastic Differentiation by Monoubiquitinating KLF5.; Authors - J Fu
mouse	WB	J Cell Mol Med; WWP2 regulates SIRT1-STAT3 acetylation and phosphorylation involved in hypertensive angiopathy.; Authors - Ying Zhang