Willin Polyklonaler Antikörper

Willin Polyklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 21039-1-AP

Synonyme

FRMD6, EX1, FERM domain containing 6, FERM domain-containing protein 6

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  WB analysis using 21039-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21039-1-AP (Willin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 21039-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21039-1-AP (Willin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 21039-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21039-1-AP (Willin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 21039-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21039-1-AP (Willin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 21039-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21039-1-AP (Willin antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of A431 using 21039-1-AP

  1X10^6 A431 cells were stained with .2ug Willin antibody (21039-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

21039-1-AP bindet in WB, IHC, FC (Intra), ELISA Willin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Willin fusion protein Ag15375
• Vollständiger Name: FERM domain containing 6
• Berechnetes Molekulargewicht: 622 aa, 72 kDa
• Beobachtetes Molekulargewicht: 72, 70, 30 kDa
• GenBank-Zugangsnummer: BC029870
• Gene symbol: FRMD6/Willin
• Gene ID (NCBI): 122786
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Willin, also known as FRMD6 or human Expanded (hEx), is a member of the FERM-domain family of cytoskeletal crosslinkers. It is a potent inhibitor of cell proliferation, transformation and modulator of drug sensitivity, suggesting it possesses tumor suppressor properties. Willin is widely expressed in normal tissues. Although willin is found in the cytoplasm and membrane, it localizes to the nuclei of almost all tumors.This antibody was raised against the C-terminal region of human FRMD6 protein and is expected to recognize the endogenous FRMD6.

Publikationen

Species	Application	Title
human	IHC	BMC Cancer; TP53 mutation status and consensus molecular subtypes of colorectal cancer in patients from Rwanda; Authors - Augustin Nzitakera