XBP1S/XBP1U Polyklonaler Antikörper

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• KD/KO Validated

XBP1S/XBP1U Polyklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, FC (Intra), ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Rezensionen (1)

Kat-Nr. : 24168-1-AP

Synonyme

XBP1, X-box-binding protein 1, luminal form, X-box-binding protein 1, cytoplasmic form, X-box-binding protein 1, X box binding protein 1

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  WB analysis of HEK-293 using 24168-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24168-1-AP (XBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 24168-1-AP

  WB result of XBP1S/XBP1U antibody (24168-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-XBP1S/XBP1U transfected HepG2 cells.

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  WB analysis using 24168-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24168-1-AP (XBP1S/XBP1U antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 24168-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24168-1-AP (XBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24168-1-AP (XBP1S/XBP1U antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24168-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24168-1-AP (XBP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 24168-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XBP1S/XBP1U (24168-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, Jurkat-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• ChIP: See 1 publications below

Produktinformation

24168-1-AP bindet in WB, IHC, FC (Intra), ChIP, ELISA XBP1S/XBP1U und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: XBP1S/XBP1U fusion protein Ag21454
• Vollständiger Name: X-box binding protein 1
• Berechnetes Molekulargewicht: 261 aa, 29 kDa
• Beobachtetes Molekulargewicht: ~32 kDa; ~60 kDa
• GenBank-Zugangsnummer: BC000938
• Gene symbol: XBP1
• Gene ID (NCBI): 7494
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

XBP1 is also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation (PMID: 14559994). IRE1α possesses both kinase and ribonuclease activity and processes XBP1 mRNA to produce an active transcription factor in response to ER stress (PMID: 11779464, 11780124). It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum, the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1. The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1S, which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1U, is constitutively expressed, and thought to function as a negative feedback regulator of XBP1S, which shuts off transcription of target genes during the recovery phase of ER stress (PMID: 11850408). The unspliced XBP1U isoform is composed of 261 amino acid residues, and the spliced XBP1S isoform is composed of 376 amino acid residues. The XBP1 antibody (24168-1-AP) can detect both XBP1U and XBP1S.

Publikationen

Species	Application	Title
rat	WB,IHC	J Hazard Mater; GRP78/IRE1 and cGAS/STING pathway crosstalk through CHOP facilitates iodoacetic acid-mediated testosterone decline; Authors - Li Mou
human	WB	J Ethnopharmacol; Network pharmacology, molecular docking, and in vitro experimental verification of the mechanism of Guanxining in treating diabetic atherosclerosis; Authors - Wenting Wang
mouse	WB	Bioorg Chem; Dihydroartemisinin alleviates steatosis and inflammation in nonalcoholic steatohepatitis by decreasing endoplasmic reticulum stress and oxidative stress.; Authors - Tongxi Li
human	WB	J Virol; Inositol requiring enzyme 1α promotes Zika virus infection through regulating stearoyl-CoA desaturase 1 mediated lipid metabolism.; Authors - Yanxia Huang ; KD Validated
mouse	WB	Exp Ther Med; Apelin‑13 reduces high glucose‑induced mitochondrial dysfunction in cochlear hair cells by inhibiting endoplasmic reticulum stress; Authors - Zhiqiang Huo
mouse	WB	J Zhejiang Univ Sci B; Endoplasmic reticulum stress is involved in retinal injury induced by repeated transient spikes of intraocular pressure; Authors - Xue YANG

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Carly (Verified Customer) (09-27-2019)

Used in an antibody array to identify proteins in human EDTA plasma.

• Applications: Other,