XPA Polyklonaler Antikörper

XPA Polyklonal Antikörper für FC, IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 16462-1-AP

Synonyme

XP1, XPA, XPAC

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Galerie der Validierungsdaten

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  WB analysis of L02 using 16462-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16462-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16462-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16462-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 16462-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16462-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16462-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16462-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 16462-1-AP

  IP result of anti-XPA (IP:16462-1-AP, 4ug; Detection:16462-1-AP 1:300) with L02 cells lysate 3000 ug.

• 

  IF Staining of HepG2 using 16462-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16462-1-AP (XPA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 16462-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XPA (16462-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: L02-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 2 publications below

Produktinformation

16462-1-AP bindet in WB, IP, IF, FC, ELISA XPA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: XPA fusion protein Ag9536
• Vollständiger Name: xeroderma pigmentosum, complementation group A
• Berechnetes Molekulargewicht: 374aa,42 kDa; 273aa,31 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC014965
• Gene symbol: XPA
• Gene ID (NCBI): 7507
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

XPA, also named as DNA repair protein complementing XP-A cells, is a 272 amino acid protein, which belongs to the XPA family. XPA is expressed in various cell lines and in skin fibroblasts. XPA is involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. XPA is required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. The calculated molecular weight of XPA is 31 kDa, but modified XPA protein is about 40 kDa (PMID: 17848622).

Publikationen

Species	Application	Title
human	WB	Front Oncol; PMID: 31249811; Authors - Ma-Sha Huang
human	WB	J Cancer; PMID: 31772670; Authors - Li-Ming Tan
mouse	WB	Genes Dev; TRF1 uses a noncanonical function of TFIIH to promote telomere replication; Authors - Zhe Yang
human,mouse	WB,IF	Adv Healthc Mater; Macromolecular NO-Donor Micelles for Targeted and Augmented Chemotherapy against Prostate Cancer; Authors - Jiaxin Chen
human	WB	Front Pharmacol; Triptolide enhances carboplatin-induced apoptosis by inhibiting nucleotide excision repair (NER) activity in melanoma; Authors - Geng Wang
zebrafish	IF	Sci Total Environ; AHR-mediated DNA damage contributes to BaP-induced cardiac malformations in zebrafish; Authors - Hongmei Zou