XPO6 Polyklonaler Antikörper

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• KD/KO Validated

XPO6 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 11408-1-AP

Synonyme

EXP6, exportin 6, KIAA0370, Ran binding protein 20, RANBP20, XPO6

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 11408-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11408-1-AP (XPO6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11408-1-AP

  IP Result of anti-XPO6 (IP:11408-1-AP, 4ug; Detection:11408-1-AP 1:300) with HeLa cells lysate 4000ug.

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  IHC staining of human heart using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11408-1-AP (XPO6 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11408-1-AP (XPO6 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11408-1-AP (XPO6 antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human kidney using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11408-1-AP (XPO6 antibody) at dilution of 1:25 (under 40x lens).

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  IHC staining of human placenta using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11408-1-AP (XPO6 antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human placenta using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11408-1-AP (XPO6 antibody) at dilution of 1:25 (under 40x lens).

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  IHC staining of human skin using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11408-1-AP (XPO6 antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human skin using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11408-1-AP (XPO6 antibody) at dilution of 1:25 (under 40x lens).

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  IHC staining of human ovary using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 11408-1-AP (XPO6 antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human ovary using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 11408-1-AP (XPO6 antibody) at dilution of 1:25 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 2 publications below

Produktinformation

11408-1-AP bindet in WB, IP, IHC, IF XPO6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, drosophila
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: XPO6 fusion protein Ag1898
• Vollständiger Name: exportin 6
• Berechnetes Molekulargewicht: 1125 aa, 129 kDa
• Beobachtetes Molekulargewicht: 128 kDa
• GenBank-Zugangsnummer: BC014071
• Gene symbol: XPO6
• Gene ID (NCBI): 23214
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human XPO6 gene encodes a Ran-binding protein 20, exportin-6, belonging to the exportin family. Exportin-6 shuttles between the nucleus and the cytoplasm and mediates the nuclear export of actin and profilin-actin complexes in somatic cells. Interaction of exportin-6 and actin is achieved in a RanGTP-dependent manner, actin is a major cytoskeletal element which is normally kept cytoplasmic by exportin-6 driven nuclear export.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.; Authors - Sergi Marco
Drosophila	IF	Proc Natl Acad Sci U S A; Active maintenance of nuclear actin by importin 9 supports transcription.; Authors - Dopie Joseph J
human	WB	Cancer Res; Genomic profiling of microRNA and messenger RNA reveals deregulated microRNA expression in prostate cancer.; Authors - Ambs Stefan S
human	WB	J Virol; The XPO6 Exportin Mediates Herpes Simplex Virus 1 gM Nuclear Release Late in Infection.; Authors - Hugo Boruchowicz ; KD Validated
human	WB	RNA; An RNA guanine quadruplex regulated pathway to TRAIL-sensitization by DDX21.; Authors - Ewan K S McRae
human	WB	JCI Insight; Potent antitumor effects of cell-penetrating peptides targeting STAT3 axis.; Authors - Maryam Aftabizadeh