XRCC2 Monoklonaler Antikörper

XRCC2 Monoklonal Antikörper für FC, IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3D9A1

Kat-Nr. : 66652-1-Ig

Synonyme

DKFZp781P0919, DNA repair protein XRCC2, XRCC2

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 66652-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66652-1-Ig (XRCC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66652-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66652-1-Ig (XRCC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66652-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66652-1-Ig (XRCC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 66652-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66652-1-Ig (XRCC2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66652-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XRCC2 antibody (66652-1-Ig, Clone: 3D9A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HepG2 using 66652-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XRCC2 (66652-1-Ig, Clone:3D9A1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, NIH/3T3-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66652-1-Ig bindet in WB, IF, FC, ELISA XRCC2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: XRCC2 fusion protein Ag14421
• Vollständiger Name: X-ray repair complementing defective repair in Chinese hamster cells 2
• Berechnetes Molekulargewicht: 280 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC042137
• Gene symbol: XRCC2
• Gene ID (NCBI): 7516
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

XRCC2, also named as DNA repair protein XRCC2, is a 280 amino acid protein, which belongs to the RecA family. XRCC2 is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. XRCC2 is a part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway.

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011