YES1-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

YES1-Specific Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 20243-1-AP

Synonyme

c yes, HsT441, P61 YES, Proto oncogene c Yes, Yes, YES1, YES1-Specific

---

Galerie der Validierungsdaten

• 

  WB analysis using 20243-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20243-1-AP (YES1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20243-1-AP

  WB result of YES1-Specific antibody (20243-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-YES1-Specific transfected HepG2 cells.

• 

  IP experiment of A549 using 20243-1-AP

  IP result of anti-YES1-Specific(IP:20243-1-AP, 4ug; Detection:20243-1-AP 1:500) with A549 cells lysate 1400 ug.

• 

  IHC staining of human lung cancer using 20243-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20243-1-AP (YES1-Specific antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20243-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20243-1-AP (YES1-Specific antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 20243-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20243-1-AP (YES1-Specific antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 20243-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20243-1-AP (YES1-Specific antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, DU 145-Zellen, HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• IHC: See 2 publications below

Produktinformation

20243-1-AP bindet in WB, IP, IHC, ELISA YES1-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: NM_005433
• Gene symbol: YES1
• Gene ID (NCBI): 7525
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YES1, also named as p61-Yes and YES, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. YES1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. The antibody is specific to YES1.

Publikationen

Species	Application	Title
human	IHC	Am J Respir Crit Care Med; YES1 Drives Lung Cancer Growth and Progression and Predicts Sensitivity to Dasatinib.; Authors - Irati Garmendia
human,mouse		Sci Adv; A brain proteomic signature of incipient Alzheimer's disease in young APOE ε4 carriers identifies novel drug targets.; Authors - Jackson A Roberts
human	IHC	J Immunother Cancer; SRC family kinase (SFK) inhibitor dasatinib improves the antitumor activity of anti-PD-1 in NSCLC models by inhibiting Treg cell conversion and proliferation.; Authors - Esther Redin ; KO Validated
human		Cell Death Dis; Linc01133 contributes to gastric cancer growth by enhancing YES1-dependent YAP1 nuclear translocation via sponging miR-145-5p.; Authors - Yujing Sun
human		J Cell Biochem; miR-17-5p up-regulates YES1 to modulate the cell cycle progression and apoptosis in ovarian cancer cell lines.; Authors - Lan Li ; KD Validated
human		iScience; DAB2IP suppresses invadopodia formation through destabilizing ALK by interacting with USP10 in breast cancer; Authors - Qingwen Huang