YTHDC1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

YTHDC1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, RIP, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Rezensionen (1)

Kat-Nr. : 29441-1-AP

Synonyme

KIAA1966, Putative splicing factor YT521, YT521, YT521 B, YTH domain containing 1, YTHDC1

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Galerie der Validierungsdaten

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  WB analysis using 29441-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29441-1-AP (YTHDC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29441-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29441-1-AP (YTHDC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 29441-1-AP

  WB result of YTHDC1 antibody (29441-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDC1 transfected HEK-293 cells.

• 

  WB analysis of HepG2 using 29441-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 29441-1-AP (YTHDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 29441-1-AP

  IP result of anti-YTHDC1(IP:29441-1-AP, 4ug; Detection:29441-1-AP 1:2000) with HEK-293 cells lysate 1800 ug.

• 

  IHC staining of human colon cancer using 29441-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29441-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 29441-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29441-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 29441-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29441-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, MDA-MB-231-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

29441-1-AP bindet in WB, RIP, IP, IHC, IF, ELISA YTHDC1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: YTHDC1 fusion protein Ag31459
• Vollständiger Name: YTH domain containing 1
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: ~100 kDa
• GenBank-Zugangsnummer: BC053863
• Gene symbol: YTHDC1
• Gene ID (NCBI): 91746
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YTHDC1, containing 1 YTH domain, is one of the m6A-binding proteins that can recognize and bind to the m6A methylation site and plays a specific role in gene expression. YTHDC1 is constitutively enriched in the nucleus. It regulates mRNA splicing by bridging the interactions between the trans- and cis-regulatory elements to bind targeted mRNAs. YTHDC1 mediates the export of m6A modified mRNA from the nucleus to the cytoplasm by interacting with SRSF3, which is an essential factor driving the tumorigenic process in various types of cancers including breast cancer, colon cancer, ovarian cancer, osteosarcoma, and glioblastoma (32368386).

Publikationen

Species	Application	Title
human	WB	Cancer Cell Int; YTHDC1-mediated VPS25 regulates cell cycle by targeting JAK-STAT signaling in human glioma cells.; Authors - Xiaolong Zhu ; KD Validated
mouse	WB	J Clin Lab Anal; Mettl3 promotes oxLDL-mediated inflammation through activating STAT1 signaling.; Authors - Zhenwei Li
human	WB	Nanoscale Res Lett; RNA m6A Alterations Induced by Biomineralization Nanoparticles: A Proof-of-Concept Study of Epitranscriptomics for Nanotoxicity Evaluation.; Authors - Jinbin Pan
human	WB	Discov Oncol; Systematic analysis of the expression profile and prognostic significance of m6A regulators and PD-L1 in hepatocellular carcinoma; Authors - Fanhua Kong
human	RIP	Cell Stem Cell; Decoding m6A RNA methylome identifies PRMT6-regulated lipid transport promoting AML stem cell maintenance; Authors - Ying Cheng
human	WB,IHC,IP	Exp Mol Med; The YTHDC1/GLUT3/RNF183 axis forms a positive feedback loop that modulates glucose metabolism and bladder cancer progression; Authors - Bin Yan ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Zhongwen (Verified Customer) (09-25-2023)

Clear band around 100KD

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

YTHDC1 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:29441-1-AP)