YTHDC2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

YTHDC2 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

20

Rezensionen (2)

Kat-Nr. : 27779-1-AP

Synonyme

YTH domain containing 2, YTHDC2

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Galerie der Validierungsdaten

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  WB analysis using 27779-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27779-1-AP (YTHDC2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27779-1-AP

  WB result of YTHDC2 antibody (27779-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDC2 transfected HepG2 cells.

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  WB analysis of HeLa using 27779-1-AP

  WB result of YTHDC2 antibody (27779-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDC2 transfected HeLa cells.

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  IHC staining of rat testis using 27779-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 27779-1-AP (YTHDC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 27779-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 27779-1-AP (YTHDC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 27779-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using YTHDC2 antibody (27779-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 27779-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human YTHDC2 (27779-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen
• Erfolgreiche Detektion in IHC: Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 17 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

27779-1-AP bindet in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA YTHDC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: YTHDC2 fusion protein Ag26690
• Vollständiger Name: YTH domain containing 2
• Beobachtetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: BC137285
• Gene symbol: YTHDC2
• Gene ID (NCBI): 64848
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YTHDC2, also named as Probable ATP-dependent RNA helicase YTHDC2, is a 1430 amino acid protein, which belongs to the DEAD box helicase family. YTHDC2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.

Publikationen

Species	Application	Title
human	IF	Mol Cell; Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.; Authors - Hagai Marmor-Kollet
human	WB	Sci Adv; Regulation of telomere homeostasis and genomic stability in cancer by N 6-adenosine methylation (m6A).; Authors - Ji Hoon Lee
human	WB	Nat Commun; Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.; Authors - Shujie Chen
human	WB,RIP	Cancer Lett; LIMK1 m6A-RNA methylation recognized by YTHDC2 induces 5-FU chemoresistance in colorectal cancer via endoplasmic reticulum stress and stress granule formation; Authors - Lu Chen ; KD Validated
mouse	WB,IP	Genes Dev; YTHDC2 control of gametogenesis requires helicase activity but not m6A binding.; Authors - Yuhki Saito
human	WB	Carcinogenesis; Genetic variants in m6A modification genes are associated with esophageal squamous-cell carcinoma in the Chinese population.; Authors - Nan Yang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Maialen (Verified Customer) (02-10-2022)

YTHDC2 was immunoprecipitated from 4 million HCT15 cells lysate using 1μg 27779-1-AP (YTHDC2 antibody). Same amount of normal rabbit IgG was used as negative control. For IP confirmation, western blot with 27779-1-AP (YTHDC2 antibody) was perfomed at 1:1000 dilution incubated overnight at 4ºC. 10% of the whole cell lysate was loaded as input.

• Applications: Western Blot, Immunoprecipitation
• Cell Tissue Type: HCT15

YTHDC2 Antibody Western Blot,Immunoprecipitation validation ( dilution) in HCT15 (Cat no:27779-1-AP)

FH

Robert (Verified Customer) (01-24-2019)

1. scrambled siRNA2. YTHDC2 siRNA12. YTHDC2 siRNA22. YTHDC2 siRNA3

• Applications: Western Blot,
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: ST cells (porcine)

YTHDC2 Antibody Western Blot, validation (1/500 dilution) in ST cells (porcine) (Cat no:27779-1-AP)