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  • KD/KO Validated

YTHDC2 Polyklonaler Antikörper

YTHDC2 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(36)

Kat-Nr. : 27779-1-AP

Synonyme

3'-5' RNA helicase YTHDC2, EC:3.6.4.13, hYTHDC2, YTH domain-containing protein 2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, HeLa-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHCRattenhodengewebe, humanes Magenkrebsgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen
Erfolgreiche Detektion in FC (Intra)HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:8000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:1000-1:4000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

27779-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA YTHDC2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen YTHDC2 fusion protein Ag26690
Vollständiger Name YTH domain containing 2
Beobachtetes Molekulargewicht 160 kDa
GenBank-ZugangsnummerBC137285
Gene symbol YTHDC2
Gene ID (NCBI) 64848
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YTHDC2, also named as Probable ATP-dependent RNA helicase YTHDC2, is a 1430 amino acid protein, which belongs to the DEAD box helicase family. YTHDC2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for YTHDC2 antibody 27779-1-APProtokoll herunterladen
IHC protocol for YTHDC2 antibody 27779-1-APProtokoll herunterladenl
IF protocol for YTHDC2 antibody 27779-1-APProtokoll herunterladen
IP protocol for YTHDC2 antibody 27779-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Cell Mol Immunol

Inhibition of METTL3 in macrophages provides protection against intestinal inflammation

Authors - Huilong Yin
humanIF

Mol Cell

Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.

Authors - Hagai Marmor-Kollet
humanWB

Sci Adv

Regulation of telomere homeostasis and genomic stability in cancer by N 6-adenosine methylation (m6A).

Authors - Ji Hoon Lee
humanWB

Nat Commun

Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.

Authors - Shujie Chen
humanWB,RIP

Cancer Lett

LIMK1 m6A-RNA methylation recognized by YTHDC2 induces 5-FU chemoresistance in colorectal cancer via endoplasmic reticulum stress and stress granule formation

Authors - Lu Chen
  • KD Validated
mouseWB,IP

Genes Dev

YTHDC2 control of gametogenesis requires helicase activity but not m6A binding.

Authors - Yuhki Saito

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Nikolett (Verified Customer) (07-23-2025)

I used this antibofy as 1:1000 for Western Blot, 1:300 for IHC and IF. Worked well without background, after overnight AB incubation in 3% BSA/PBS for WB. For IHC and IF I used NaCi pH6 for antigene retrieval and it worked nicely.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:1000 for Western Blot, 1:300 for IHC and IF
  • Cell Tissue Type: Human Lung Cancer Patient Samples
FH

Maialen (Verified Customer) (02-10-2022)

YTHDC2 was immunoprecipitated from 4 million HCT15 cells lysate using 1μg 27779-1-AP (YTHDC2 antibody). Same amount of normal rabbit IgG was used as negative control. For IP confirmation, western blot with 27779-1-AP (YTHDC2 antibody) was perfomed at 1:1000 dilution incubated overnight at 4ºC. 10% of the whole cell lysate was loaded as input.

  • Applications: Western Blot, Immunoprecipitation
  • Cell Tissue Type: HCT15
YTHDC2 Antibody Western Blot,Immunoprecipitation validation ( dilution) in HCT15 (Cat no:27779-1-AP)
FH

Robert (Verified Customer) (01-24-2019)

1. scrambled siRNA2. YTHDC2 siRNA12. YTHDC2 siRNA22. YTHDC2 siRNA3

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: ST cells (porcine)
YTHDC2 Antibody Western Blot, validation (1/500 dilution) in ST cells (porcine) (Cat no:27779-1-AP)