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YY1 Monoklonaler Antikörper
YY1 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
Affe, human, Maus, Ratte und mehr (1)
Anwendung
WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA
Konjugation
Unkonjugiert
CloneNo.
2E11C5
Kat-Nr. : 66281-1-Ig
Synonyme
"YY1 Antibodies" Comparison
View side-by-side comparison of YY1 antibodies from other vendors to find the one that best suits your research needs.
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | C6-Zellen, A431-Zellen, COS-7-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen |
| Erfolgreiche IP | NIH/3T3-Zellen |
| Erfolgreiche Detektion in IHC | humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Tonsillitisgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | HepG2-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:5000-1:20000 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
Produktinformation
66281-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA YY1 und zeigt Reaktivität mit Affe, human, Maus, Ratten
| Getestete Reaktivität | Affe, human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Hausschwein, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG2a |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | YY1 fusion protein Ag17732 |
| Vollständiger Name | YY1 transcription factor |
| Berechnetes Molekulargewicht | 414 aa, 45 kDa |
| Beobachtetes Molekulargewicht | 65-70 kDa |
| GenBank-Zugangsnummer | BC037308 |
| Gene symbol | YY1 |
| Gene ID (NCBI) | 7528 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for YY1 antibody 66281-1-Ig | Protokoll herunterladen |
| IHC protocol for YY1 antibody 66281-1-Ig | Protokoll herunterladenl |
| IF protocol for YY1 antibody 66281-1-Ig | Protokoll herunterladen |
| IP protocol for YY1 antibody 66281-1-Ig | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Cell Pervasive Chromatin-RNA Binding Protein Interactions Enable RNA-Based Regulation of Transcription. | ||
Mol Ther Nucleic Acids Angiotensin II-induced muscle atrophy via PPARγ suppression is mediated by miR-29b. | ||
Cell Death Dis CircHIPK3 promotes colorectal cancer growth and metastasis by sponging miR-7. | ||
Cancers (Basel) MiR-302b as a Combinatorial Therapeutic Approach to Improve Cisplatin Chemotherapy Efficacy in Human Triple-Negative Breast Cancer. |



















