ZCCHC6 Polyklonaler Antikörper

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• KD/KO Validated

ZCCHC6 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 25196-1-AP

Synonyme

HS2, KIAA1711, PAPD6, Terminal uridylyltransferase 7, TUT7, TUTase 7, ZCCHC6

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Galerie der Validierungsdaten

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  WB analysis of PC-3 using 25196-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25196-1-AP (ZCCHC6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25196-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25196-1-AP (ZCCHC6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25196-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25196-1-AP (ZCCHC6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 25196-1-AP

  IP Result of anti-ZCCHC6 (IP:25196-1-AP, 4ug; Detection:25196-1-AP 1:1000) with HeLa cells lysate 1800ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HEK-293T-Zellen, Maushirngewebe
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

25196-1-AP bindet in WB, IP, IF, CoIP, ELISA ZCCHC6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZCCHC6 fusion protein Ag18240
• Vollständiger Name: zinc finger, CCHC domain containing 6
• Berechnetes Molekulargewicht: 1495 aa, 171 kDa
• Beobachtetes Molekulargewicht: 171 kDa
• GenBank-Zugangsnummer: BC032456
• Gene symbol: ZCCHC6
• Gene ID (NCBI): 79670
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZCCHC6 also named as KIAA1711 ,HS2 or TUT7 is a 1495 amino acid protein, which contains threes CCHC-type zinc fingers and two PAP-associated domains. A member of the DNA polymerase type-B-like family, ZCCHC6 as a uridylyltransferase that mediates RNA uridylation.

Publikationen

Species	Application	Title
human	IF	Mol Cell; Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.; Authors - Hagai Marmor-Kollet
human	WB	Mol Cell; 3' Uridylation Confers miRNAs with Non-canonical Target Repertoires.; Authors - Acong Yang ; KO Validated
human	WB	Mol Cell; A Mechanism for microRNA Arm Switching Regulated by Uridylation.; Authors - Haedong Kim ; KD Validated
human	WB,IP	Nat Commun; TENT2, TUT4, and TUT7 selectively regulate miRNA sequence and abundance; Authors - Acong Yang
human	WB,CoIP	Nat Commun; AGO-bound mature miRNAs are oligouridylated by TUTs and subsequently degraded by DIS3L2.; Authors - Acong Yang
human	WB	Elife; The molecular basis of coupling between poly(A)-tail length and translational efficiency.; Authors - Kehui Xiang

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