ZEB1 Polyklonaler Antikörper

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• KD/KO Validated

ZEB1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF, IP, CoIP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen(270)

Rezensionen (2)

Kat-Nr. : 21544-1-AP

Synonyme

AREB6, NIL2A, NIL-2-A zinc finger protein, TCF8, TCF-8

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  WB analysis using 21544-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21544-1-AP (ZEB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21544-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21544-1-AP (ZEB1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21544-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21544-1-AP (ZEB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 21544-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21544-1-AP (ZEB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 21544-1-AP

  IP result of anti-ZEB1 (IP:21544-1-AP, 4ug; Detection:21544-1-AP 1:8000) with COLO 320 cells lysate 1320 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, COLO 320-Zellen, MCF-7-Zellen, MDA-MB-453s-Zellen, Maus-Kolongewebe, PC-3-Zellen
• Erfolgreiche IP: COLO 320-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 23 publications below
• WB: See 245 publications below
• IHC: See 33 publications below
• IF: See 22 publications below
• IP: See 5 publications below
• CoIP: See 4 publications below
• ChIP: See 13 publications below

Produktinformation

21544-1-AP bindet in WB, IHC, IF, IP, CoIP, chIP, ELISA ZEB1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZEB1 fusion protein Ag16204
• Vollständiger Name: zinc finger E-box binding homeobox 1
• Berechnetes Molekulargewicht: 1125 aa, 124 kDa
• Beobachtetes Molekulargewicht: 190-210 kDa
• GenBank-Zugangsnummer: BC112392
• Gene symbol: ZEB1
• Gene ID (NCBI): 6935
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZEB1(zinc-finger-enhancer binding protein 1), which is a member of the Zinc finger-Homeobox (ZFH) family of repressors, acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. Depending on the quantity of cDNA and on the cell type, ZEB1 can enhance or repress the promoter activity of the ATP1A1 gene. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. The calculated molecular weight of ZEB1 is 124 kDa, but the post-phosphorylation of protein is about 190-210 kDa. This is a rabbit polyclonal antibody raised against part of the full length of ZEB1 of human.

Publikationen

Species	Application	Title
mouse	IHC	Adv Sci (Weinh); The Conserved LncRNA DIO3OS Restricts Hepatocellular Carcinoma Stemness by Interfering with NONO-Mediated Nuclear Export of ZEB1 mRNA; Authors - Ya-Rui Hou
human	chIP	Nat Commun; A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure.; Authors - Yufeng Yao
human	WB	Nat Commun; Long non-coding RNA NR2F1-AS1 induces breast cancer lung metastatic dormancy by regulating NR2F1 and ΔNp63.; Authors - Yingjie Liu
human	WB,IP,ChIP	Nat Commun; Cooperative interaction between ERα and the EMT-inducer ZEB1 reprograms breast cancer cells for bone metastasis.; Authors - Nastaran Mohammadi Ghahhari
human	WB,ChIP	J Exp Med; Squamous cell carcinoma subverts adjacent histologically normal epithelium to promote lateral invasion.; Authors - Priyanka Singh
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kathryn (Verified Customer) (10-14-2021)

Antibody worked really well first time and produced clear Zeb1 bands at the MW expected, with barely any non specific binding.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HUVECs

FH

K (Verified Customer) (12-20-2020)

I used tis Ab for western blotting at 1:2000 dilution and got good results.

• Applications: Western Blot
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: human liver cells