ZEB1 Monoklonaler Antikörper

ZEB1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

CloneNo.

1H1F1

Kat-Nr. : 66279-1-Ig

Synonyme

1H1F1, AREB6, NIL2A, NIL-2-A zinc finger protein, TCF8

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  WB analysis using 66279-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66279-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 66279-1-Ig

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66279-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66279-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66279-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66279-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66279-1-Ig (ZEB1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66279-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66279-1-Ig (ZEB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66279-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66279-1-Ig (ZEB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66279-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66279-1-Ig (ZEB1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 66279-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZEB1 antibody (66279-1-Ig, Clone: 1H1F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IF: See 1 publications below

Produktinformation

66279-1-Ig bindet in WB, IHC, IF/ICC, ELISA ZEB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ZEB1 fusion protein Ag17118
• Vollständiger Name: zinc finger E-box binding homeobox 1
• Berechnetes Molekulargewicht: 1125 aa, 124 kDa
• Beobachtetes Molekulargewicht: 190-210 kDa
• GenBank-Zugangsnummer: BC112392
• Gene symbol: ZEB1
• Gene ID (NCBI): 6935
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZEB1(zinc-finger-enhancer binding protein 1), which is a member of the Zinc finger-Homeobox (ZFH) family of repressors, acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. Depending on the quantity of cDNA and on the cell type, ZEB1 can enhance or repress the promoter activity of the ATP1A1 gene. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. The calculated molecular weight of ZEB1 is 124 kDa, but the post-phosphorylation of protein is about 190-210 kDa.

Publikationen

Species	Application	Title
human	WB	Theranostics; FSH induces EMT in ovarian cancer via ALKBH5-regulated Snail m6A demethylation; Authors - Xingyan Xu
human	WB	Biochim Biophys Acta Mol Cell Res; The calcium pump PMCA4 prevents epithelial-mesenchymal transition by inhibiting NFATc1-ZEB1 pathway in gastric cancer.; Authors - Teng Wang
human	WB	Breast Cancer; Overexpression of SERPINA3 promotes tumor invasion and migration, epithelial-mesenchymal-transition in triple-negative breast cancer cells.; Authors - Yingzi Zhang
human	WB	J Clin Med; A Mutation in ZNF143 as a Novel Candidate Gene for Endothelial Corneal Dystrophy.; Authors - Yonggoo Kim
human	WB	Front Oncol; Identification of hub genes within the CCL18 signaling pathway in hepatocellular carcinoma through bioinformatics analysis; Authors - Jinlei Mao
mouse	IF	Am J Physiol Lung Cell Mol Physiol; NFkB/RelA Signaling in Secretoglobin Progenitors Mediates Plasticity and MMP-Induced Barrier Disruption In House Dust Mite-Induced Allergic Asthma; Authors - Melissa E Skibba