SARA Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SARA Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 22033-1-AP

Synonyme

hSARA, Madh interacting protein, MADHIP, Novel serine protease, NSP, Receptor activation anchor, SARA, SMADIP, ZFYVE9

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Galerie der Validierungsdaten

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  WB analysis using 22033-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22033-1-AP (SARA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 22033-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22033-1-AP (SARA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 22033-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22033-1-AP (SARA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 22033-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22033-1-AP (SARA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22033-1-AP

  IP Result of anti-SARA (IP:22033-1-AP, 5ug; Detection:22033-1-AP 1:1000) with mouse brain tissue lysate 5000ug.

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  IHC staining of human kidney using 22033-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 22033-1-AP (SARA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 22033-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 22033-1-AP (SARA antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse brain using 22033-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SARA antibody (22033-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse kidney using 22033-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using SARA antibody (22033-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, humanes Hirngewebe, Maushirngewebe, MCF-7-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe, Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Produktinformation

22033-1-AP bindet in WB, IP, IHC, IF, ELISA SARA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SARA fusion protein Ag16929
• Vollständiger Name: zinc finger, FYVE domain containing 9
• Berechnetes Molekulargewicht: 1425 aa, 156 kDa
• Beobachtetes Molekulargewicht: 156 kDa
• GenBank-Zugangsnummer: BC032680
• Gene symbol: ZFYVE9
• Gene ID (NCBI): 9372
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZFYVE9, also known as SARA (Smad anchor for receptor activation), is a double zinc finger (FYVE domain) protein that interacts directly with SMAD2 and SMAD3, and is involved in Alzheimer's disease. SARA functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. SARA plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. SARA is possibly associated with TGF-beta receptor internalization.

Publikationen

Species	Application	Title
human	WB	Structure; Structural Basis for Specific Interaction of TGFβ Signaling Regulators SARA/Endofin with HD-PTP.; Authors - Deepankar Gahloth ; KD Validated
mouse	WB,IF	J Bone Miner Res; Dysfunction of Caveolae-mediated Endocytic TβRI Degradation Results in Hypersensitivity of TGF-β/Smad Signaling in Osteogenesis Imperfecta; Authors - Changgui Shi
human	WB,IF	Sci Adv; Neuropilin 1 is an entry receptor for KSHV infection of mesenchymal stem cell through TGFBR1/2-mediated macropinocytosis; Authors - Zheng-Zhou Lu ; KO Validated