ZO-1 Rekombinanter Antikörper
ZO-1 Rekombinant Antikörper für IF/ICC, FC (Intra)
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human
Anwendung
IF/ICC, FC (Intra)
Konjugation
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2P13
Kat-Nr. : CL488-82870
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in IF/ICC | U2OS-Zellen |
Erfolgreiche Detektion in FC (Intra) | MCF-7-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:300-1:1200 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
CL488-82870 bindet in IF/ICC, FC (Intra) ZO-1 und zeigt Reaktivität mit human
Getestete Reaktivität | human |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Rekombinant |
Typ | Antikörper |
Immunogen | ZO-1 fusion protein Ag33182 |
Vollständiger Name | tight junction protein 1 (zona occludens 1) |
Berechnetes Molekulargewicht | 1748 aa, 195 kDa |
Beobachtetes Molekulargewicht | 230 kDa |
GenBank-Zugangsnummer | BC111712 |
Gene symbol | ZO-1 |
Gene ID (NCBI) | 7082 |
Konjugation | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signaling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
---|---|
IF protocol for CL Plus 488 ZO-1 antibody CL488-82870 | Protokoll herunterladen |
FC protocol for CL Plus 488 ZO-1 antibody CL488-82870 | Download protocol |
STANDARD-PROTOKOLLE | |
---|---|
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |